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p21 Cip1抗体 [GT1032]

KO/KD Orthogonal
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Flow cytometry (FACS). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in IHC (Paraffin sections) (IHC-P). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

目录号 GTX629543

文献引用 21
4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT1032

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P, FACS, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

18 kDa. ( Note )

阳性对照

HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)

预测种属反应

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888121

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

细胞定位

Cytoplasm , Nucleus

功能与背景

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

KO/KD

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr)
C. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 48 hr)
15% SDS-PAGE
p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p21 Cip1 antibody [GT1032] used in Immunoprecipitation (IP). GTX629543

GTX629543 IP Image

p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
A. 30 μg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of CDKN1A protein by 4 μg p21 Cip1 antibody [GT1032] (GTX629543)
15 % SDS-PAGE
The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

Orthogonal

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543

GTX629543 ICC/IF Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.
Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p21 Cip1 antibody [GT1032] used in Flow cytometry (FACS). GTX629543

GTX629543 FACS Image

p21 Cip1 antibody [GT1032] (GTX629543) detects p21 Cip1 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-p21 Cip1 antibody [GT1032] used in IHC (Paraffin sections) (IHC-P). GTX629543

GTX629543 IHC-P Image

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543

GTX629543 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25961745

文献引用

Application Reference

Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5) : 993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
实验应用 : WB
种属反应 : Human
Zanr? V et al. Int J Mol Sci. 2022; 23 (3) : 1121 Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
实验应用 : WB
种属反应 : Human
Ya-Hui Chen et al. Cancers (Basel) 2021; 13 (18)
实验应用 : WB
种属反应 : Human
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产品评论

p21 Cip1 antibody [GT1032] 目录号 GTX629543

Rating
( Average 4.5 based on 4 users reviews)
实验应用
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 14-Oct-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 22°C, 0.5Hr
Primary Antibody : 1:2000, 22°C, 18Hr
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 13-Mar-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : KOPN79 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-p21 Cip1 antibody [GT1032] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629543
Date : Anonymous submitted on 03-Jan-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MIA PaCa-2 cells
Blocking : 3% BSA, 25°C, 0.3Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.8% Triton X-100 in 3% BSA
Primary Antibody : 1:100, 4°C, 16Hr
Anti-p21 Cip1 antibody [GT1032] used in Western Blot (WB). GTX629543
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCT116 cells
Amount used : 30μg
Blocking : 1%BSA in PBST, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
我要评论产品
Application Reference
Hsueh YH et al. J Orthop Translat 2023; 38:141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5):993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
实验应用 : WB
种属反应 : Human
Zanr? V et al. Int J Mol Sci. 2022; 23 (3):1121 Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
实验应用 : WB
种属反应 : Human
Ching-Ling Lin et al. Biomol Ther (Seoul) 2021; 29 (5):551-561 Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo.
实验应用 : WB
种属反应 : Human
Ya-Hui Chen et al. Cancers (Basel) 2021; 13 (18)
实验应用 : WB
种属反应 : Human
Pons BJ et al. Cell Mol Life Sci 2021; 78 (17-18):6319-6335 Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response.
实验应用 : WB
种属反应 : Human
Li R et al. Invest Ophthalmol Vis Sci 2021; 62 (9):2 Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway.
实验应用 : ICC/IF
种属反应 : Human
Chen YH et al. Cancer Manag Res 2020; 12:4645-4665
实验应用 : WB
种属反应 : Human
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
实验应用 : WB
种属反应 : Human
Yu S et al. Int Immunopharmacol 2020; 81:106294 M1 macrophages accelerate renal glomerular endothelial cell senescence through reactive oxygen species accumulation in streptozotocin-induced diabetic mice.
实验应用 : WB
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
实验应用 : WB
种属反应 : Human
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells.
实验应用 : WB
种属反应 : Human
Chen KY et al. PLoS One 2019; 14 (7):e0219317 Resveratrol induced premature senescence and inhibited epithelial-mesenchymal transition of cancer cells via induction of tumor suppressor Rad9.
实验应用 : WB
种属反应 : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
实验应用 : WB
种属反应 : Mouse
Hsu KW et al. Int J Mol Sci 2018; 19 (2) The Application of Non-Invasive Apoptosis Detection Sensor (NIADS) on Histone Deacetylation Inhibitor (HDACi)-Induced Breast Cancer Cell Death.
Huang LC et al. Dis Markers 2018; 2018:3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
Tian YF et al. Am J Cancer Res 2018; 8 (9):1812-1822 Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
实验应用 : WB
种属反应 : Human
Huang L.C. et al. Disease Markers 2018; 2018:Article ID 3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
实验应用 : WB
种属反应 : Human
Chang TC et al. PLoS One 2015; 10 (5):e0126537 High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy.
实验应用 : WB
种属反应 : Human
Chung CL et al. Evid Based Complement Alternat Med 2015; 2015:595824 Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.
实验应用 : WB
种属反应 : Mouse
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PBS.pdf
产品包装 报价 (¥)
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¥ 1700
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