mCherry抗体

Name: mCherry抗体
Brand: GeneTex
SKU: GTX128508
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-mCherry antibody used in IHC (Whole mount) (IHC-Wm). GTX128508

Anti-mCherry antibody used in Western Blot (WB). GTX128508

Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128508

Anti-mCherry antibody used in Immunoprecipitation (IP). GTX128508

Anti-mCherry antibody used in IHC (Frozen sections) (IHC-Fr). GTX128508

目录号 GTX128508

文献引用 54

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-Fr, IHC-Wm, IP, PLA
种属反应	Species independent
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 mCherry 的产品

View product citations for antibody GTX128508 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent
IHC-Wm	1:100-1:500
IP	1:100-1:500
PLA	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Full length mCherry recombinant protein

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2721247

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX128508 IHC-Wm Image

mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.

GTX128508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128508 ICC/IF Image

mCherry antibody detects mCherry protein at mCherry fusion protein by immunofluorescent analysis.
Sample: mCherry fusion protein-transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 30 min, then Triton X-100 for 1 min.
Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
Red: mCherry is expressed in the transfected cell . Blue: Hoechst 33342 staining.

GTX128508 WB Image

mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody (GTX128508) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128508 IP Image

mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX128508 ICC/IF Image

The data was published in the journal Nat Commun in 2017.PMID: 29203870

GTX128508 ICC/IF Image

The data was published in the journal EMBO J in 2019.PMID: 31368584

GTX128508 IHC-Fr Image

The data was published in the journal Nat Commun in 2017.PMID: 28466843

GTX128508 IHC-Fr Image

The data was published in the journal Nat Commun in 2017.PMID: 28466843

GTX128508 ICC/IF Image

The data was published in the journal Nat Commun in 2017.PMID: 29203870

GTX128508 WB Image

The data was published in the journal Front Cell Dev Biol in 2020.PMID: 32457908

GTX128508 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

GTX128508 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31908141

文献引用

Application Reference

SM de Leeuw et al. STAR Protoc 2023; 5 (1) : 102779 Intracellular cholesterol visualization in brain tissue using D4H

Corno A et al. EMBO J 2023; 42 (20) : e112630 A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.

Clarke JP et al. Front Mol Biosci 2023; 10 : 1178439 Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.

Yang ZH et al. BMC Med 2023; 21 (1) : 254 Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.

Longarini EJ et al. Mol Cell 2023; 83 (10) : 1743-1760.e11 Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.

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产品评论

mCherry antibody 目录号 GTX128508

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 21-Jul-2021

Score :

Application Tested : WB

Sample : Plasma of protein X-mCherry knockin mouse

Blocking : 5% skim milk, 25°C, 1Hr

Primary Antibody : 1:1000, 25°C, 1Hr

Date : Anonymous submitted on 02-Nov-2018

Score :

Application Tested : IHC-Wm

Sample : Drosophila Brain

Blocking : 10% NGS in 0.3% PBST, 25°C, 4Hr

Fixation : 4% PFA, 4°C, overnightHr

Primary Antibody : 1:500, 4°C, 16Hr

我要评论产品

Application Reference

SM de Leeuw et al. STAR Protoc 2023; 5 (1):102779 Intracellular cholesterol visualization in brain tissue using D4H

Corno A et al. EMBO J 2023; 42 (20):e112630 A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.

Clarke JP et al. Front Mol Biosci 2023; 10:1178439 Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.

Yang ZH et al. BMC Med 2023; 21 (1):254 Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS locus mediating dendritic spine morphogenesis and cognitive function.

Longarini EJ et al. Mol Cell 2023; 83 (10):1743-1760.e11 Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.

Dabrowski R et al. J Cell Biol 2023; 222 (7) Parallel phospholipid transfer by Vps13 and Atg2 determines autophagosome biogenesis dynamics.

Weng M et al. J Virol 2023; 97 (3):e0154522 Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation.

实验应用 : WB

Galer EL et al. J Neurosci 2023; 43 (3):419-432 Cervical Epidural Electrical Stimulation Increases Respiratory Activity through Somatostatin-Expressing Neurons in the Dorsal Cervical Spinal Cord in Rats.

Preston AJ et al. Cell Death Discov 2023; 9 (1):66 Elephant TP53-RETROGENE 9 induces transcription-independent apoptosis at the mitochondria.

实验应用 : WB, ICC/IF

Buijs TJ et al. EMBO J 2023; 42 (3):e111348 STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons.

实验应用 : ICC/IF

Feringa FM et al. J Neurosci 2023; 43 (3):347-358 An Atypical, Staged Cell Death Pathway Induced by Depletion of SNARE-Proteins MUNC18-1 or Syntaxin-1.

实验应用 : ICC/IF

Cocozza F et al. EMBO J 2023; 42 (24):e113590 Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic?cells.

Guh CY et al. Nat Commun 2022; 13 (1):5781 XPF activates break-induced telomere synthesis.

实验应用 : WB

Su W et al. Nat Commun 2022; 13 (1):6577 Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice.

实验应用 : WB, IP

Fechner J et al. Int J Mol Sci 2022; 23 (20) The Binding of CSL Proteins to Either Co-Activators or Co-Repressors Protects from Proteasomal Degradation Induced by MAPK-Dependent Phosphorylation.

Rui Chen et al. Genome Med 2022; 14 (1):53 Functional genomic analysis delineates regulatory mechanisms of GWAS-identified bipolar disorder risk variants.

实验应用 : ICC/IF

Banta KL et al. Immunity 2022; 55 (3):512-526.e9 Mechanistic convergence of the TIGIT and PD-1 inhibitory pathways necessitates co-blockade to optimize anti-tumor CD8+ T?cell responses.

实验应用 : WB

Buser DP et al. Life Sci Alliance 2022; 5 (7) Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.

Wang L et al. Microbiol Spectr 2022;:e0186021 Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.

实验应用 : WB

Toni Beltran et al. EMBO J 2021; 40 (5):e105564 Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors.

实验应用 : IHC-Fr

Ahmet C Berkyurek et al. EMBO J 2021; 40 (5):e105565 The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription.

van Berkel AA et al. J Neurochem 2021; 157 (3):450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.

Chassefeyre R et al. Sci Adv 2021; 7 (52):eabg3693 Endosomal sorting drives the formation of axonal prion protein endoggresomes.

实验应用 : WB

Nader GPF et al. Cell 2021; 184 (20):5230-5246.e22 Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion.

Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.

实验应用 : IHC-Wm

Nagashima S et al. J Biol Chem 2021; 297 (1):100803 CSE1L promotes nuclear accumulation of transcriptional coactivator TAZ and enhances invasiveness of human cancer?cells.

Corrales RM et al. PLoS Pathog 2021; 17 (6):e1009666 The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host.

Suzuki S et al. Mol Cell Biochem 2021; 476 (4):1673-1690 DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.

Yuki Miura et al. Nat Biotechnol 2020; 38 (12):1421-1430 Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.

Wang C et al. Front Cell Dev Biol 2020; 8:329 PCDH7 Inhibits the Formation of Homotypic Cell-in-Cell Structure.

Wang LT et al. EMBO Rep 2020; 21 (2):e48795 PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition.

Melick CH et al. J Biol Chem 2020; 295 (23):8096-8105 A-kinase anchoring protein 8L interacts with mTORC1 and promotes cell growth.

实验应用 : WB

Sim?es FC et al. Nat Commun 2020; 11 (1):600 Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair.

实验应用 : IHC-Fr

Yang Z et al. Mol Psychiatry 2020; 25 (1):48-66 The genome-wide risk alleles for psychiatric disorders at 3p21.1 show convergent effects on mRNA expression, cognitive function, and mushroom dendritic spine.

Pan X et al. Sci Rep 2019; 9 (1):19110 FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.

实验应用 : WB

Smith RJ et al. Cell Rep 2019; 28 (8):2206-2219.e8 PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.

实验应用 : ICC/IF

Miyagawa T et al. J Cell Biol 2019; 218 (10):3355-3371 MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane.

实验应用 : PLA

Chang CH et al. Cell Rep 2019; 28 (5):1195-1205.e3 The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice.

实验应用 : IHC-Fr

Brouwer M et al. EMBO J 2019;:e101289 SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering.

实验应用 : ICC/IF

Ikeda KN et al. J Biol Chem 2019; 294 (30):11486-11497 Spatial proteomics reveal that the protein phosphatase PTP1B interacts with and may modify tyrosine phosphorylation of the rhomboid protease RHBDL4.

Shahror RA et al. J Neurotrauma 2019; (Epub) Transplantation of Mesenchymal Stem Cells Overexpressing FGF21 Facilitates Cognitive Recovery and Enhances Neurogenesis in a Mouse Model of Traumatic Brain Injury.

实验应用 : IHC-Fr

Jewell JL et al. Elife 2019; 8 GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor.

Richard J Smith et al. bioRxiv 2019; (Epub) PP1 and PP2A use opposite phospho-dependencies to control distinct processes at the kinetochore.

Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

实验应用 : WB

Enya S et al. Dis Model Mech 2018; 11 (8) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.

Stewart SE et al. J Cell Sci 2018; 131 (14) Transbilayer phospholipid movement facilitates the translocation of annexin across membranes.

Zhang Q et al. Cell 2018; 174 (4):870-883.e17 The Mitochondrial Unfolded Protein Response Is Mediated Cell-Non-autonomously by Retromer-Dependent Wnt Signaling.

Yao X et al. Dev Cell 2018; 45 (4):526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.

实验应用 : IHC-Fr

Stewart SE et al. bioRxiv 2018; (Epub) Phospholipid flipping facilitates annexin translocation across membranes.

实验应用 : WB

Taulet N et al. Nat Commun 2017; 8 (1):1928 IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

实验应用 : ICC/IF

Pfefferli C et al. Nat Commun 2017; 8:15151 The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin.

Yao X et al. Cell Res 2017; 27 (6):801-814 Homology-mediated end joining-based targeted integration using CRISPR/Cas9.

实验应用 : IHC-Fr

Yang FM et al. Biochem J 2015; 471 (2):199-209 A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding.

实验应用 : WB

Chou CM et al. J Biomed Sci 2015; 22:102 Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish.

实验应用 : WB

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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mCherry抗体

目录号 GTX128508

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

产品属性

形式

存储溶液

存放说明

浓度

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

研究领域

產品图片

GTX128508 IHC-Wm Image

GTX128508 WB Image

GTX128508 WB Image

GTX128508 ICC/IF Image

GTX128508 WB Image

GTX128508 IP Image

GTX128508 ICC/IF Image

GTX128508 ICC/IF Image

GTX128508 IHC-Fr Image

GTX128508 IHC-Fr Image

GTX128508 ICC/IF Image

GTX128508 WB Image

GTX128508 WB Image

GTX128508 WB Image

文献引用

Application Reference

产品评论

mCherry antibody 目录号 GTX128508

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