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mCherry抗体 [GT857]

Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

目录号 GTX630189

宿主

Mouse

克隆

Monoclonal

克隆编号

GT857

同种型

IgG1

实验应用

WB, ICC/IF, IHC-Wm, IHC

种属反应

Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Wm Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

mCherry-tagged protein expressing 293T cells

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Full length mCherry recombinant protein

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888193

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

GTX630189 WB Image

mCherry antibody [GT857] detects mCherry protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells
5% SDS-PAGE
mCherry antibody [GT857] (GTX630189) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

产品评论

mCherry antibody [GT857] 目录号 GTX630189

Rating ( Average 5 based on 3 users reviews)
实验应用
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Red: GTX630189 mcherry antibody: medulla, Green: GTX113617 GFP antibody: photoreceptor cell R8
Date : Anonymous submitted on 18-Jul-2017
Score :
Application Tested : IHC
Sample : Drosophila Brain tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : red: mcherry 543 nm (overexpression) ; megenta: anti-mouse with cy5
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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