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iNOS抗体

Orthogonal
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

目录号 GTX130246

文献引用 35
3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 iNOS 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

131 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886221

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

功能与背景

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

数据库

研究领域

產品图片

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in Western Blot (WB). GTX130246

GTX130246 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

Orthogonal

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: Mock and treated Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects Inos protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Inos stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in Western Blot (WB). GTX130246

GTX130246 WB Image

Orthogonal

The data was published in the journal Front Cell Neurosci in 2018.PMID: 30483062

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25105760

文献引用

Application Reference

Zhao J et al. J Cell Mol Med 2024; 28 (7) : e18172 The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis.
Ishigooka G et al. J Clin Med 2023; 12 (20) Effects of Angiotensin Receptor Blockers on Streptozotocin-Induced Diabetic Cataracts.
Brusini R et al. Pharmaceutics 2023; 15 (7) Assessment of Squalene-Adenosine Nanoparticles in Two Rodent Models of Cardiac Ischemia-Reperfusion.
Teramoto H et al. Cells 2023; 12 (14) Calcineurin B1 Deficiency Reduces Proliferation, Increases Apoptosis, and Alters Secretion in Enteric Glial Cells of Mouse Small Intestine in Culture.
P Wu et al. Mil Med Res 2023; 10 (1) : 35 The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine
实验应用 : ICC/IF
种属反应 : Mouse
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产品评论

iNOS antibody 目录号 GTX130246

Rating
( Average 4.7 based on 3 users reviews)
实验应用
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 02-Nov-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells treated with 1 ug/ml LPS for 24 h
Blocking : Skim milk, RT°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 11-Aug-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Bone marrow derived macrophage
Amount used : 20 μg
Blocking : 3% BSA+0.05% Tween20 in PBS, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
条带说明 : Bone marrow derived macrophage (M0) differentiated to M1 by LPS stimulation.
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% nonfat dry milk, 28°C, 0.5Hr
Primary Antibody : 1:1000, 28°C, 1Hr
条带说明 : Lane 1: RAW264.7 cells, Lane 2: RAW264.7 cells (1 ug/ml LPS treatment for 24 hours)
我要评论产品
Application Reference
Zhao J et al. J Cell Mol Med 2024; 28 (7):e18172 The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis.
Ishigooka G et al. J Clin Med 2023; 12 (20) Effects of Angiotensin Receptor Blockers on Streptozotocin-Induced Diabetic Cataracts.
Brusini R et al. Pharmaceutics 2023; 15 (7) Assessment of Squalene-Adenosine Nanoparticles in Two Rodent Models of Cardiac Ischemia-Reperfusion.
Teramoto H et al. Cells 2023; 12 (14) Calcineurin B1 Deficiency Reduces Proliferation, Increases Apoptosis, and Alters Secretion in Enteric Glial Cells of Mouse Small Intestine in Culture.
P Wu et al. Mil Med Res 2023; 10 (1):35 The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine
实验应用 : ICC/IF
种属反应 : Mouse
Zhang J et al. Pharmaceuticals (Basel) 2023; 16 (5) GRK2 Mediates Macrophage Polarization by Regulating EP4-cAMP-pCREB Signaling in Ulcerative Colitis and the Therapeutic Effect of Paroxetine on Mice with DSS-Induced Colitis.
Shi Y et al. J Neuroinflammation 2023; 20 (1):91 N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1.
实验应用 : WB
种属反应 : Mouse
Huang HC et al. Int J Mol Sci 2022; 23 (13) Effects of PCSK-9 Inhibition by Alirocumab Treatments on Biliary Cirrhotic Rats.
Hsin-Tzu Liu et al. Antioxidants (Basel) 2022; 11 (5) Baicalein Exerts Therapeutic Effects against Endotoxin-Induced Depression-like Behavior in Mice by Decreasing Inflammatory Cytokines and Increasing Brain-Derived Neurotrophic Factor Levels.
实验应用 : WB
种属反应 : Mouse
Lin ZH et al. J Ethnopharmacol 2022; 282:114636 Water extract of medicinal ink (WEMI) attenuates lipopolysaccharide-induced NO production of Raw264.7 cells via downregulating JAK2/STAT3-mediated iNOS expression.
实验应用 : WB
种属反应 : Mouse
Cai M et al. CNS Neurosci Ther 2021; 27 (11):1348-1365 Sevoflurane preconditioning protects experimental ischemic stroke by enhancing anti-inflammatory microglia/macrophages phenotype polarization through GSK-3β/Nrf2 pathway.
实验应用 : ICC/IF
Anyaegbu CC et al. J Neurosci Methods 2021; 359:109223 Simultaneous flow cytometric characterization of multiple cell types and metabolic states in the rat brain after repeated mild traumatic brain injury.
种属反应 : Rat
Ohira H et al. PLoS One 2021; 16 (2):e0246580 Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer.
实验应用 : IHC-P
种属反应 : Mouse
Yang Y et al. Biomaterials 2021; 264:120390 Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage apoptosis and Re-polarization.
种属反应 : Mouse
Tingting Wang et al. Front Cell Dev Biol 2020; 8:593890 DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.
实验应用 : WB
种属反应 : Rat
Scherwitzl I et al. Mol Ther Oncolytics 2020; 17:431-447 Sindbis Virus with Anti-OX40 Overcomes the Immunosuppressive Tumor Microenvironment of Low-Immunogenic Tumors.
Ekova MR et al. Bull Exp Biol Med 2020; 169 (5):718-720 Peculiarities of the Expression of Inducible NO Synthase in Rat Dentate Gyrus in Depression Modeling.
实验应用 : IHC-P
种属反应 : Rat
Zhou Y et al. Oncol Lett 2020; 20 (6):298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
实验应用 : WB
种属反应 : Mouse
Zhu S et al. J Agric Food Chem 2020; 68 (42):11726-11734 Collagen Hydrolysate Corrects Anemia in Chronic Kidney Disease via Anti-Inflammatory Renoprotection and HIF-2α-Dependent Erythropoietin and Hepcidin Regulation.
实验应用 : WB
种属反应 : Mouse
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
实验应用 : WB
种属反应 : Mouse
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
实验应用 : WB
种属反应 : Rat
Arturo A Wilkins-Rodr?guez et al. Infect Immun 2020; 88 (7) Differential Regulation of l-Arginine Metabolism through Arginase 1 during Infection with Leishmania mexicana Isolates Obtained from Patients with Localized and Diffuse Cutaneous Leishmaniasis.
实验应用 : WB
种属反应 : Mouse
Zhang HK et al. J Mol Neurosci 2020; 70 (4):618-630 Gypenosides Prevent H2O2-Induced Retinal Ganglion Cell Apoptosis by Concurrently Suppressing the Neuronal Oxidative Stress and Inflammatory Response.
实验应用 : WB
种属反应 : Rat
Morita N et al. Microbes Infect 2020; (Epub) Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages.
实验应用 : WB
种属反应 : Mouse
Zhang D et al. Nature 2019; 574 (7779):575-580 Metabolic regulation of gene expression by histone lactylation.
Fukuda M et al. J Neurosurg 2019;:1-13 Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation.
实验应用 : WB
Ghosh R et al. Int J Biol Macromol 2019; 139:221-232 Panax quinquefolius (North American ginseng) cell suspension culture as a source of bioactive polysaccharides: Immunostimulatory activity and characterization of a neutral polysaccharide AGC1.
实验应用 : WB
种属反应 : Mouse
Barati S et al. J Cell Biochem 2019; (Epub) Mesenchymal stem cell mediated effects on microglial phenotype in cuprizone-induced demyelination model.
实验应用 : IHC-P
种属反应 : Mouse
Jiao M et al. Chemosphere 2019; 226:678-686 Effect of the SSeCKS-TRAF6 interaction on gastrodin-mediated protection against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced astrocyte activation and neuronal death.
实验应用 : WB
种属反应 : Mouse
Su WJ et al. Front Cell Neurosci 2018; 12:412 Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.
实验应用 : WB
种属反应 : Mouse
Chao W et al. Int Immunopharmacol 2017; 50:77-86 3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
实验应用 : WB
种属反应 : Mouse
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
实验应用 : WB
种属反应 : Mouse
Mei S et al. PLoS One 2015; 10 (8):e0135867 MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN and FOG2 Expression.
实验应用 : WB
种属反应 : Mouse
Zhao X et al. PLoS One 2014; 9 (8):e104506 PI3K/Akt signaling pathway modulates influenza virus induced mouse alveolar macrophage polarization to M1/M2b.
实验应用 : ICC/IF
种属反应 : Mouse
Yang YC et al. Antioxid Redox Signal 2013; 18 (10):1129-40 Topoisomerase II-mediated DNA cleavage and mutagenesis activated by nitric oxide underlie the inflammation-associated tumorigenesis.
实验应用 : WB
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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