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beta Actin抗体 [AC-15]

KO/KD
Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

目录号 GTX26276

文献引用 60

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 AC-15

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IP, ELISA

种属反应

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Monkey, Carp, Hirudo medicinalis
产品包装
100 μl (¥5600)
查看所有 beta Actin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5,000-1:10,000
ICC/IF 1:1,000-1:2,000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

阳性对照

human or chicken fibroblast

产品属性

形式

Liquid

存储溶液

Ascites

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Xenopus laevis

免疫原

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_367161

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actin beta , BRWS1 , PS1TP5BP1

细胞定位

Cytoplasm, cytoskeleton,Nucleus

功能与背景

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

数据库

研究领域

產品图片

Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276

GTX26276 IHC-P Image

IHC-P analysis of human heart tissue using GTX26276 beta Actin antibody [AC-15] at 1:1,000.

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of HS-68 cells using GTX26276 beta Actin antibody [AC-15] at 1:1000 (green) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Actin antibody [AC-15] at 1:5,000.

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of FS-11 cells using GTX26276 beta Actin antibody [AC-15].

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

KO/KD

The data was published in the journal Front Mol Neurosci in 2017.PMID: 29163025

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Biomedicines in 2020. PMID: 32244938

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal J Neurosci in 2017. PMID: 28724750

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 29691463

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the 2022 in Stem Cell Res Ther. PMID: 35477424

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the 2022 in Stem Cell Res Ther. PMID: 35477424

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 31480758

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31448224

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27848974

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

The data was published in the journal Front Mol Neurosci in 2017.PMID: 29163025

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal PLoS One in 2013.PMID: 23675505

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31640125

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

The data was published in the journal Front Mol Neurosci in 2017.PMID: 29163025

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31640125

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Front Mol Neurosci in 2018.PMID: 29910711

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Cancer Cell Int in 2016.PMID: 27418879

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31640125

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27876799

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31640125

文献引用

Application Reference

MJ Young et al. Cell Death Differ 2024; USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy
实验应用 : WB
Dujardin E et al. Elife 2023; 12 DMRT1 is a testis-determining gene in rabbits and is also essential for female fertility.
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Hammersen T et al. Cells 2023; 12 (12) Inverse Regulation of Cartilage Neogenesis at Physiologically Relevant Calcium Conditions by Human Articular Chondrocytes and Mesenchymal Stromal Cells.
Hennlein L et al. J Cell Biol 2023; 222 (3) Plastin 3 rescues cell surface translocation and activation of TrkB in spinal muscular atrophy.
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Application Reference
MJ Young et al. Cell Death Differ 2024; USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy
实验应用 : WB
Dujardin E et al. Elife 2023; 12 DMRT1 is a testis-determining gene in rabbits and is also essential for female fertility.
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Hammersen T et al. Cells 2023; 12 (12) Inverse Regulation of Cartilage Neogenesis at Physiologically Relevant Calcium Conditions by Human Articular Chondrocytes and Mesenchymal Stromal Cells.
Hennlein L et al. J Cell Biol 2023; 222 (3) Plastin 3 rescues cell surface translocation and activation of TrkB in spinal muscular atrophy.
Madan V et al. EMBO J 2023; 42 (23):e114473 HEATR5B associates with dynein-dynactin and promotes motility of AP1-bound endosomal membranes.
Klampfleuthner FAM et al. Cells 2022; 11 (19) Stage-Dependent Activity and Pro-Chondrogenic Function of PI3K/AKT during Cartilage Neogenesis from Mesenchymal Stromal Cells.
实验应用 : WB
种属反应 : Human
Jessica Giehrl-Schwab et al. EMBO Mol Med 2022; 14 (5):e14797 Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons.
实验应用 : WB
Janine L?ckgen et al. Stem Cell Res Ther 2022; 13 (1):168 NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels.
实验应用 : WB
Matthias Gerstner et al. Int J Mol Sci 2021; 22 (7) Heparan Sulfate Deficiency in Cartilage: Enhanced BMP-Sensitivity, Proteoglycan Production and an Anti-Apoptotic Expression Signature after Loading.
实验应用 : WB
种属反应 : Mouse
Yanjun Liu et al. Redox Biol 2021; 43:102002 Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition.
实验应用 : WB
种属反应 : Mouse
Chasan S et al. Advanced Functional Materials 2021; (Epub) Sulfation of Glycosaminoglycan Hydrogels Instructs Cell Fate and Chondral versus Endochondral Lineage Decision of Skeletal Stem Cells In Vivo.
实验应用 : WB
种属反应 : Human
Ghanawi H et al. Nucleic Acids Res 2021; 49 (21):12284-12305 Loss of full-length hnRNP R isoform impairs DNA damage response in motoneurons by inhibiting Yb1 recruitment to chromatin.
实验应用 : WB
种属反应 : Mouse
Raman V et al. Nat Commun 2021; 12 (1):6116 Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases.
实验应用 : WB
Teng YN et al. Cells 2021; 10 (6) Etoposide Triggers Cellular Senescence by Inducing Multiple Centrosomes and Primary Cilia in Adrenocortical Tumor Cells.
实验应用 : WB
种属反应 : Mouse
Chen TY et al. Cell Death Differ 2021; (Epub) Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.
实验应用 : WB
种属反应 : Human
Vikram Babu Kasaragod et al. Proc Natl Acad Sci U S A 2020; 117 (52):33235-33245 Pyridoxal kinase inhibition by artemisinins down-regulates inhibitory neurotransmission.
实验应用 : WB
种属反应 : Human
Tanaka K et al. PLoS One 2020; 15 (9):e0238604 Difference in pyruvic acid metabolism between neonatal and adult mouse lungs exposed to hyperoxia.
Melnik S et al. Stem Cell Res Ther 2020; 11 (1):532 MiR-218 affects hypertrophic differentiation of human mesenchymal stromal cells during chondrogenesis via targeting RUNX2, MEF2C, and COL10A1.
实验应用 : WB
种属反应 : Human
Dreher SI et al. Front Cell Dev Biol 2020; 8:81 Significance of MEF2C and RUNX3 Regulation for Endochondral Differentiation of Human Mesenchymal Progenitor Cells.
Kreuser U et al. Front Cell Dev Biol 2020; 8:581331 Initial WNT/β-Catenin Activation Enhanced Mesoderm Commitment, Extracellular Matrix Expression, Cell Aggregation and Cartilage Tissue Yield From Induced Pluripotent Stem Cells.
实验应用 : WB
种属反应 : Human
Vikram Babu Kasaragod et al. bioRxiv 2020; (Epub) Pyridoxal Kinase Inhibition by Artemisinins Downregulates Inhibitory Neurotransmission.
实验应用 : WB
Nagano N et al. Biomedicines 2020; 8 (3) Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression.
实验应用 : IP
种属反应 : Mouse
Saha S et al. Front Oncol 2019; 9:692 Transcriptomic Analysis Identifies RNA Binding Proteins as Putative Regulators of Myelopoiesis and Leukemia.
实验应用 : WB
种属反应 : Human
Wang CY et al. Int J Mol Sci 2019; 20 (20) Fetuin-A Inhibits Placental Cell Growth and Ciliogenesis in Gestational Diabetes Mellitus.
实验应用 : WB
种属反应 : Human
Buchert J et al. Int J Mol Sci 2019; 20 (17) The Role of Extracellular Matrix Expression, ERK1/2 Signaling and Cell Cohesiveness for Cartilage Yield from iPSCs.
实验应用 : WB
种属反应 : Human
Claes Z et al. J Biol Chem 2019; The antibiotic robenidine exhibits guanabenz-like cytoprotective properties by a mechanism independent of protein phosphatase PP1:PPP1R51A.
实验应用 : WB
种属反应 : Human
Diederichs S et al. Cell Mol Life Sci 2019; (Epub) Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy.
实验应用 : WB
种属反应 : Mouse
Colussi DJ et al. SLAS Discov 2019; 24 (3):295-303 Patient-Derived Phenotypic High-Throughput Assay to Identify Small Molecules Restoring Lysosomal Function in Tay-Sachs Disease.
实验应用 : WB
种属反应 : Human
Schaefer N et al. Front Mol Neurosci 2018; 11:167 Functional Consequences of the Postnatal Switch From Neonatal to Mutant Adult Glycine Receptor α1 Subunits in the Shaky Mouse Model of Startle Disease.
Chen TY et al. J Cell Physiol 2018; (Epub) Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued.
实验应用 : WB
种属反应 : Human
Bhattacharjee P et al. Sci Rep 2018; 8 (1):6458 Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses.
实验应用 : WB
种属反应 : Mouse
Romero M et al. Cell Death Differ 2017; 24 (5):798-808 Intrinsic repair protects cells from pore-forming toxins by microvesicle shedding.
Dombert B et al. Front Mol Neurosci 2017; 10:346 BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).
实验应用 : ICC/IF
种属反应 : Mouse
Shan YS et al. Oncol Lett 2017; 14 (4):4517-4526 Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer.
Moradi M et al. J Cell Biol 2017; 216 (3):793-814 Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons.
Lin RK et al. Oncotarget 2017; 8 (69):113431-113443 Hypermethylation of BEND5 contributes to cell proliferation and is a prognostic marker of colorectal cancer.
实验应用 : WB
种属反应 : Human
Shan YS et al. Oncol Lett 2017; (Epub) Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer.
实验应用 : WB
种属反应 : Human
Schaefer N et al. J Neurosci 2017; 37 (33):7948-7961 Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.
实验应用 : WB
种属反应 : Mouse
He C et al. Prostate 2017; 77 (11):1187-1198 Characterization of a novel p110β-specific inhibitor BL140 that overcomes MDV3100-resistance in castration-resistant prostate cancer cells.
实验应用 : WB
种属反应 : Human
Mohammadreza Zamanian et al. Cancer Cell Int 2016; 16:56 Calreticulin mediates an invasive breast cancer phenotype through the transcriptional dysregulation of p53 and MAPK pathways.
实验应用 : WB
种属反应 : Human
Ikink GJ et al. Nat Commun 2016; 7:13567 IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation.
实验应用 : WB
种属反应 : Human
Dexheimer V et al. Sci Rep 2016; 6:36655 Differential expression of TGF-β superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation.
实验应用 : WB
种属反应 : Human
Fischer J et al. JCP 2016; (Epub) Role of PTHrP(1-34) Pulse Frequency Versus Pulse Duration to Enhance Mesenchymal Stromal Cell Chondrogenesis.
实验应用 : WB
种属反应 : Human
Tseng YY et al. J Virol 2015; 89 (22):11619-29 Regulation of PACT-Mediated Protein Kinase Activation by the OV20.0 Protein of Orf Virus.
Bramanti V et al. J Neurosci Res 2015; 93 (9):1378-87 modulation of extracellular signal-related kinase, cyclin d1, glial fibrillary acidic protein, and vimentin expression in *-pretreated astrocyte cultures treated with competence and progression growth factors.
实验应用 : WB
种属反应 : Rat
Hamacher-Brady A et al. J Biol Chem 2015; 290 (36):22005-18 Bax/Bak-dependent, Drp1-independent Targeting of X-linked Inhibitor of Apoptosis Protein (XIAP) into Inner Mitochondrial Compartments Counteracts Smac/DIABLO-dependent Effector Caspase Activation.
实验应用 : WB
种属反应 : Human
Tseng YY et al. J Virol 2015; 89 (9):4966-79 Functional analysis of the short isoform of orf virus protein OV20.0.
实验应用 : WB
种属反应 : Human
Hamacher-Brady A et al. Cell Death Differ 2014; 21 (12):1862-76 Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase.
Hou HH et al. Autophagy 2014; 10 (9):1509-21 Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
实验应用 : WB
种属反应 : Human
Zhu Y et al. J Biol Chem 2013; 288 (2):1099-113 Modulation of serines 17 and 24 in the LC3-interacting region of Bnip3 determines pro-survival mitophagy versus apoptosis.
实验应用 : WB
Giesert F et al. PLoS One 2013; 8 (5):e63778 Expression analysis of Lrrk1, Lrrk2 and Lrrk2 splice variants in mice.
实验应用 : WB
Unoki M et al. J Biol Chem 2013; 288 (9):6053-62 Lysyl 5-hydroxylation, a novel histone modification, by Jumonji domain containing 6 (JMJD6).
实验应用 : WB
种属反应 : Mouse
Insalaco L et al. J Cell Mol Med 2012; 16 (9):2186-95 analysis of molecular mechanisms and anti-tumoural effects of * acid in breast cancer cells.
Stelter L et al. J Nucl Med 2012; 53 (2):281-6 Novel mechanistic insights into arginine deiminase pharmacology suggest 18F-FDG is not suitable to evaluate clinical response in melanoma.
Kelly MP et al. Br J Cancer 2012; 106 (2):324-32 Arginine deiminase PEG20 inhibits growth of small cell lung cancers lacking expression of argininosuccinate synthetase.
Jablonka S et al. Exp Neurol 2011; 232 (2):261-9 therapeutic effects of pegylated *-like growth factor i in the pmn mouse model of motoneuron disease.
Franco M et al. Blood 2011; 118 (10):2906-17 Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
实验应用 : WB
Tanaka S et al. J Toxicol Sci 2010; 35 (3):327-34 Salbutamol inhibits lipopolysaccharide-induced inflammatory responses in rat peritoneal macrophages.
Bizzozero OA et al. J Neurosci Res 2009; 87 (13):358549 Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.
实验应用 : WB
种属反应 : Mouse
SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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