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alpha Tubulin抗体

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

目录号 GTX112141

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr

种属反应

Human, Mouse, Rat, Drosophila, Chicken, Caenorhabditis elegans, Mosquito, Tetrahymena
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Jurkat , Raji

预测种属反应

Sheep, Bovine, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10722892

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 μg Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (GTX112141) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Sample (30 μg of cell lysate)
A: HeLa
B: HeLa cytosol fraction
C: HeLa nucleus fraction
10% SDS PAGE
GTX112141 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31723205

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the 2019 in J Clin Med. PMID: 31121901

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 30678307

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 25950483

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Oncogene in 2017. PMID: 28436950

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 30622262

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal Cell Div in 2019. PMID: 31110557

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal Calcif Tissue Int in 2017. PMID: 28097375

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 26838546

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26161782

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25993510

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Cell Discov in 2015.PMID: 27462424

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25993510

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28676741

文献引用

产品评论

alpha Tubulin antibody 目录号 GTX112141

Rating ( Average 4.5 based on 6 users reviews)
实验应用
Western Blot(WB) ( Average 4.8 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa and MCF-7 cell lysates
Amount used : 20μg
Blocking : 5% Skim Milk in PBS, 25°C, 1Hr
Primary Antibody : 1:1000, 1°C, 25Hr
Date : Anonymous submitted on 09-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Tetrahymena
Sample : Tetrahymena
Blocking : 10% Donkey serum in PBS, 20°C, 0.5Hr
Fixation : 2% PFA in PHEM buffer (PEHEM; 60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 4 mM MgSO4, pH 6.9)
Permeabilization /Antigen retrieval : 0.5% Triton-X in PBS
Primary Antibody : 1:800, 20°C, 1Hr
Figure Note : A projection image of the whole cell was constructed from 96 optical sections obtained at 0.2 μm intervals.
Date : Anonymous submitted on 07-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco2 cells
Amount used : 25 ㎍
Blocking : 3% BSA, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 1Hr
Figure Note : Image courtesy of Nao Sugihara from National Defense Medical College.
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
条带说明 : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells,Mahlavu cells,Huh6 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : Y1 cells
Blocking : 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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