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ZO-1抗体 [HL1185]

Recombinant antibody KO/KD Orthogonal
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491
Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

目录号 GTX636491

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1185

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ZO-1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

195 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909994

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tight junction protein 1 , ZO-1

细胞定位

Cell membrane,Cell junction

功能与背景

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

GTX636491 WB Image

KO/KD

Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636491

GTX636491 ICC/IF Image

ZO-1 antibody [HL1185] detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody [HL1185] (GTX636491) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

GTX636491 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

GTX636491 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

GTX636491 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491

GTX636491 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (GTX636491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品评论

ZO-1 antibody [HL1185] 目录号 GTX636491

Rating
( Average 3 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-ZO-1 antibody [HL1185] used in Western Blot (WB). GTX636491
Date : Anonymous submitted on 22-Jan-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human corneal epithelial cells with/without drug treatment
Amount used : 30μg
Blocking : commercial blocking buffer, RT°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 20Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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