GeneTex中国官方网站
  • China

VGluT1抗体

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148
Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

目录号 GTX133148

文献引用 2
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 VGluT1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

62 kDa. ( Note )

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Recombinant protein encompassing a sequence within the Intracellular domain of mouse VGluT1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2801548

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 , 2900052E22Rik , AI851913 , Vglut1

细胞定位

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Membrane; Multi-pass membrane protein (Potential) , Cell junction , synapse , synaptosome

功能与背景

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

数据库

研究领域

產品图片

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133148

GTX133148 IHC-Fr Image

VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133148

GTX133148 IHC-P Image

VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VGluT1 antibody (GTX133148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VGluT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133148

GTX133148 ICC/IF Image

VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGluT1 stained by VGluT1 antibody (GTX133148) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VGluT1 antibody used in Western Blot (WB). GTX133148

GTX133148 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody (GTX133148) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Liu Z et al. Biomater Res 2023; 27 (1) : 108 Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation.
Seiya Yamada et al. iScience 2020; 23 (5) : 101058 Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.
实验应用 : IHC-Fr
种属反应 : Mouse
我要回报文献

产品评论

VGluT1 antibody 目录号 GTX133148

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-VGluT1 antibody used in Western Blot (WB). GTX133148
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 2.5μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 16°C, 25Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com