V5 tag抗体

Name: V5 tag抗体
Brand: GeneTex
SKU: GTX117997
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

Anti-V5 tag antibody used in ChIP assay (ChIP assay). GTX117997

目录号 GTX117997

文献引用 21

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP, ChIP assay
种属反应	Species independent
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX117997 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IP	1:100-1:500
ChIP assay	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to V5 tag

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618481

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

V5 tag antibody

研究领域

產品图片

GTX117997 IP Image

V5 tag recombinant protein was immunoprecipitated from HeLa transfected cell lysate by using V5 tag antibody (GTX117997). The precipitated V5 tag recomabinant was detected by GTX117997 diluted at 1:1000. A: Input B: IP by V5 tag antibody

GTX117997 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody (GTX117997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX117997 ICC/IF Image

V5 tag antibody detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody (GTX117997) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX117997 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

GTX117997 ChIP assay Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

GTX117997 ICC/IF Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

GTX117997 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

GTX117997 ICC/IF Image

The data was published in the journal PLoS One in 2013. PMID: 23717625

GTX117997 WB Image

The data was published in the journal Nat Commun in 2019.PMID: 31171792

GTX117997 IP Image

The data was published in the journal Sci Rep in 2016.PMID: 27216889

GTX117997 IP Image

The data was published in the journal Sci Rep in 2016.PMID: 27216889

文献引用

Application Reference

Veit M et al. Int J Mol Sci 2022; 23 (21) Using Alphafold2 to Predict the Structure of the Gp5/M Dimer of Porcine Respiratory and Reproductive Syndrome Virus.

实验应用 : WB

Yan X et al. Transl Oncol 2022; 24 : 101485 Single nucleotide mutation changes the capability of CCN3 in osteosarcoma cell invasion and differentiation.

Yoann Abel et al. Nucleic Acids Res 2022; 50 (4) : 2172-2189 The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.

实验应用 : WB

Jao TM et al. Cancer Lett 2021; 499 : 290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.

实验应用 : WB

Metje-Sprink J et al. Sci Rep 2020; 10 (1) : 12956 Crystal structure of the Rab33B/Atg16L1 effector complex.

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产品评论

V5 tag antibody 目录号 GTX117997

Rating

( Average 5 based on 2 users reviews)

实验应用

Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 5 based on 1 users reviews)

Anti-V5 tag antibody used in IHC (Paraffin sections) (IHC-P). GTX117997

Date : Anonymous submitted on 19-Apr-2022

Score :

Application Tested : IHC-P

Sample : human cancer injected in mouse tissue

Blocking : 5% BSA in TBST, 25°C, 0.5Hr

Fixation : 10% formalin

Permeabilization /Antigen retrieval : 0.3% Triton lysis buffer/Sodium Citrate Buffer

Primary Antibody : 1:100, 4°C, > 12Hr

Date : Anonymous submitted on 24-Oct-2011

Score :

Application Tested : ICC/IF

Sample : 293T cells

Blocking : 3% BSA in PBS, 25°C, 1Hr

Fixation : 2% formaldehyde in PBS

Permeabilization /Antigen retrieval : 0.1% Triton X-100/PBS

Primary Antibody : 1:1000, 4°C, 16Hr

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Application Reference

Veit M et al. Int J Mol Sci 2022; 23 (21) Using Alphafold2 to Predict the Structure of the Gp5/M Dimer of Porcine Respiratory and Reproductive Syndrome Virus.

实验应用 : WB

Yan X et al. Transl Oncol 2022; 24:101485 Single nucleotide mutation changes the capability of CCN3 in osteosarcoma cell invasion and differentiation.

Yoann Abel et al. Nucleic Acids Res 2022; 50 (4):2172-2189 The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.

实验应用 : WB

Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.

实验应用 : WB

Metje-Sprink J et al. Sci Rep 2020; 10 (1):12956 Crystal structure of the Rab33B/Atg16L1 effector complex.

Hoglin BE et al. Gen Comp Endocrinol 2020; 293:113463 Pharmacological properties of whale shark (Rhincodon typus) melanocortin-2 receptor and melancortin-5 receptor: Interaction with MRAP1 and MRAP2.

Lin ST et al. Front Physiol 2019; 10:1308 Transcription Factor Elf3 Modulates Vasopressin-Induced Aquaporin-2 Gene Expression in Kidney Collecting Duct Cells.

实验应用 : WB

Bernascone I et al. Nat Commun 2019; 10 (1):2481 Sfrp3 modulates stromal-epithelial crosstalk during mammary gland development by regulating Wnt levels.

实验应用 : WB

Wolverton EA et al. Gen Comp Endocrinol 2019; 282:113215 Analyzing the signaling properties of gar (Lepisosteus oculatus) melanocortin receptors: Evaluating interactions with MRAP1 and MRAP2.

Barney E et al. Gen Comp Endocrinol 2019; 272:42-51 Elephant shark melanocortin receptors: Novel interactions with MRAP1 and implication for the HPI axis.

实验应用 : ICC/IF

Chen KR et al. Viruses 2018; 10 (12) Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease.

实验应用 : WB

Stephanie Grainger et al. bioRxiv 2018; (Epub) EGFR confers exquisite specificity of Wnt9a-Fzd9b signaling in hematopoietic stem cell development.

实验应用 : WB

Santhakumar D et al. Sci Rep 2018; 8 (1):6794 chicken *-induced protein with tetratricopeptide repeats 5 antagonizes replication of rna viruses.

实验应用 : WB

Timblin GA et al. Mol Cell Biol 2017; (Epub) Dual mechanism of Rag gene repression by c-Myb during pre-B cell proliferation.

实验应用 : WB

Chang KH et al. J Formos Med Assoc 2016; Functional properties of LRRK2 mutations in Taiwanese Parkinson disease.

实验应用 : WB

Shimizu H et al. Sci Rep 2016; 6:26618 Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits.

实验应用 : WB

Lin PH et al. J Biomed Sci 2015; 22 (1):44 N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting.

实验应用 : WB, ICC/IF, IP

Liu YJ et al. FEBS Lett 2015; 589 (4):432-9 Aberrant activation of AMP-activated protein kinase contributes to the abnormal distribution of HuR in amyotrophic lateral sclerosis.

实验应用 : ICC/IF

Wang Z et al. Proc Natl Acad Sci U S A 2014; 111 (1):E89-98 Interplay of mevalonate and Hippo pathways regulates RHAMM transcription via YAP to modulate breast cancer cell motility.

实验应用 : WB

Kinoshita Y et al. Bone 2014; 67C:145-151 Functional analysis of mutant FAM20C in Raine syndrome with FGF23-related hypophosphatemia.

实验应用 : WB

Lei L et al. PLoS One 2013; 8 (5):e64529 Identification of a novel nuclear localization signal sequence in Chlamydia trachomatis-secreted hypothetical protein CT311.

实验应用 : ICC/IF

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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V5 tag抗体

目录号 GTX117997

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX117997 IP Image

GTX117997 WB Image

GTX117997 ICC/IF Image

GTX117997 WB Image

GTX117997 ChIP assay Image

GTX117997 ICC/IF Image

GTX117997 WB Image

GTX117997 ICC/IF Image

GTX117997 WB Image

GTX117997 IP Image

GTX117997 IP Image

文献引用

Application Reference

产品评论

V5 tag antibody 目录号 GTX117997

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