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Trident pico Western HRP Substrate

Trident pico Western HRP Substrate. GTX17435
Trident pico Western HRP Substrate. GTX17435

目录号 GTX17435

文献引用 4

实验应用

 WB
产品包装
500 ml (¥3300),
100 ml (¥1200)
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产品说明

Content

Trident femto-ECL Content Package
GTX17435 (100ml) GTX17435 Trident pico Western HRP Substrate Solution A 50ml
GTX17435 Trident pico Western HRP Substrate Solution B 50ml
GTX17435 (500ml) GTX17435 Trident pico Western HRP Substrate Solution A 250ml
GTX17435 Trident pico Western HRP Substrate Solution B 250ml

 

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存放说明

Trident pico Western HRP Substrate shelf life is 24 months when stored in the dark at 2ºC to 8ºC. Keep container tightly closed. Store Trident pico Western HRP Substrate away from heat or light. Mixed substrate can be stored for up to 24 hours at 2-8ºC.

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Trident pico-ECL

功能与背景

Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.

產品图片

Trident pico Western HRP Substrate. GTX17435

GTX17435 WB Image

Serial dilutions of purified mCherry recombinant protein were prepared and applied at 10 μL/well. After electrophoresis and protein transfer, membranes were probed with anti-mCherry antibody (GTX128508) diluted at 1:2000 and then with goat anti-rabbit HRP-conjugated secondary antibody (1:2000). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate, SuperSignalTM West Femto, Trident pico Western HRP Substrate, ClarityTM, Trident plus Western HRP Substrate, and LuminataTM Forte. The blots were simultaneously exposed for 36 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system. SuperSignalTM West Femto, ClarityTM, LuminataTM Forte are registered trademark of Thermo Fisher Scientific, Bio-Rad, and EMD Millipore, respectively. The trademark holder is not affiliated with GeneTex and does not endorse this product.

Trident pico Western HRP Substrate. GTX17435

GTX17435 Image

文献引用

Application Reference

Chiu CH et al. J Biomed Sci 2023; 30 (1) : 13 ER ribosomal-binding protein 1 regulates blood pressure and potassium homeostasis by modulating intracellular renin trafficking.
Correa VSMC et al. FEBS Open Bio 2023; 13 (3) : 545-555 The NLRP3 inflammasome - interleukin 1β axis in uveal melanoma.
Lenzini L et al. Mol Genet Metab Rep 2022; 30 : 100830 A novel MRPS34 gene mutation with combined OXPHOS deficiency in an adult patient with Leigh syndrome.
Saunders IT et al. Cancer Cell Int 2019; 19 : 98 Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways.
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SDS
MSDS-GTX17435_20231219.pdf
产品包装 报价 (¥)
¥ 3300
¥ 1200
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