产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:2000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human TIMELESS. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886050
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
timeless circadian regulator , TIM , TIM1 , hTIM
细胞定位
Nucleus
功能与背景
Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
数据库
研究领域
產品图片
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GTX129604 WB Image
TIMELESS antibody detects TIMELESS protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
5 % SDS-PAGE
TIMELESS antibody (GTX129604) dilution: 1:1000
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GTX129604 WB Image
TIMELESS antibody detects TIMELESS protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TIMELESS antibody (GTX129604) dilution: 1:1000
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GTX129604 IP Image
Immunoprecipitation of TIMELESS protein from 293T whole cell extracts using 5 μg of TIMELESS antibody (GTX129604).
Western blot analysis was performed using TIMELESS antibody (GTX129604).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX129604 ICC/IF Image
TIMELESS antibody detects TIMELESS protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIMELESS protein stained by TIMELESS antibody (GTX129604) diluted at 1:2000.
Blue: Hoechst 33342 staining.
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文献引用
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