产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:50-1:200 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Huh7 (5 μM MG132) , Huh7 (10 μM MG132) , HepG2 (3μM Thapsigargin treatment for 12hr and 24hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr)
预测种属反应
Zebrafish, Sheep, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.08 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Ser403 of human SQSTM1 / P62. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885723
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B
功能与背景
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX128171 IHC-P Image
SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX128171 FACS Image
SQSTM1 (phospho Ser403) antibody (GTX128171) detects SQSTM1 (phospho Ser403) protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
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GTX128171 IHC-P Image
SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX128171 WB Image
SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract (untreated)
B. 30 μg Huh7 whole cell lysate/extract (5 μM MG132)
C. 30 μg Huh7 whole cell lysate/extract (untreated)
D. 30 μg Huh7 whole cell lysate/extract (10 μM MG132)
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128171 WB Image
HepG2 cells were untreated or treated with 3μM thapsigargin for 12 and 24 hrs. Whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 (phospho Ser403) antibody (GTX128171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128171 ICC/IF Image
SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome by immunofluorescent analysis.Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000.Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX128171 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128171 WB Image
The data was published in the journal Physiol Rep in 2017. PMID: 29263116
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GTX128171 WB Image
The data was published in the journal Physiol Rep in 2017. PMID: 29263116
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GTX128171 WB Image
The data was published in the 2019 in Cell Death Dis. PMID: 31320609
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文献引用
产品评论
SQSTM1 / P62 (phospho Ser403) antibody
目录号 GTX128171
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Rating
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( Average 4.5 based on 2 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 24-Jun-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
NG108-15 cells
Amount used :
20μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:1000, 25°C, 1.5Hr
Date :
Anonymous submitted on 08-May-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Amount used :
25μg
Blocking :
5% BSA, 25°C, 0.5Hr
Primary Antibody :
1:1000, 25°C, 18Hr
