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SOD1抗体

KO/KD Orthogonal
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659

目录号 GTX100659

文献引用 12
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

16 kDa. ( Note )

阳性对照

Rat brain , 293T , A431 , HeLa , HepG2

预测种属反应

Zebrafish, Rabbit, Sheep, Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951972

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100659 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2018. PMID: 30279365

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

文献引用

Application Reference

Ayoubi R et al. F1000Res 2023; 12 : 391 Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1?(SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.
Man-Hau Ho et al. Redox Biol 2021; 46 : 102067 CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.
实验应用 : WB
种属反应 : Mouse
Gentilin E et al. Chem Biol Interact 2021; 345 : 109575 Hydrogen peroxide toxicity on auditory cells: An in vitro study.
实验应用 : WB
Chang HM et al. Antioxidants (Basel) 2021; 10 (5) Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury.
种属反应 : Rat
Murata K et al. J Nat Med 2021; 75 (1) : 142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
实验应用 : IHC-Fr
种属反应 : Mouse
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产品评论

SOD1 antibody 目录号 GTX100659

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实验应用
Western Blot(WB)
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Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and SOD1 KO cells
Amount used : 20μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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Application Reference
Ayoubi R et al. F1000Res 2023; 12:391 Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1?(SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.
Man-Hau Ho et al. Redox Biol 2021; 46:102067 CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.
实验应用 : WB
种属反应 : Mouse
Gentilin E et al. Chem Biol Interact 2021; 345:109575 Hydrogen peroxide toxicity on auditory cells: An in vitro study.
实验应用 : WB
Chang HM et al. Antioxidants (Basel) 2021; 10 (5) Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury.
种属反应 : Rat
Murata K et al. J Nat Med 2021; 75 (1):142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
实验应用 : IHC-Fr
种属反应 : Mouse
Liu W et al. Oxid Med Cell Longev 2020; 2020:5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Tsai SH et al. FASEB J 2020; 34 (7):9498-9511 Aldehyde dehydrogenase 2 protects against abdominal aortic aneurysm formation by reducing reactive oxygen species, vascular inflammation, and apoptosis of vascular smooth muscle cells.
实验应用 : WB
种属反应 : Human
Wangta Liu et al. Oxidative Medicine and Cellular Longevity 2020; 2020:Article ID 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
实验应用 : WB
种属反应 : Human
Chou CK et al. Environ Toxicol 2019; 34 (4):401-414 Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes.
实验应用 : WB
种属反应 : Human
Chang YC et al. Int J Mol Sci 2018; 19 (10) Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells.
实验应用 : WB
种属反应 : Human
Chen HW et al. Toxicol Appl Pharmacol 2014; Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats.
实验应用 : WB
种属反应 : Rat
Tury A et al. Dev Neurobiol 2013; Altered expression of atypical PKC and Ryk in the spinal cord of a mouse model of amyotrophic lateral sclerosis.
实验应用 : WB, IHC-Fr
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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