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SDHA抗体 [GT20710]

KO/KD Orthogonal
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636
Anti-SDHA antibody [GT20710] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632636
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636
Anti-SDHA antibody [GT20710] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632636
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636
Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

目录号 GTX632636

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT20710

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SDHA 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

73 kDa. ( Note )

阳性对照

293T

预测种属反应

Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888322

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

细胞定位

Mitochondrion inner membrane

功能与背景

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636

GTX632636 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.

Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636

GTX632636 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SDHA antibody [GT20710] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632636

GTX632636 ICC/IF Image

Orthogonal

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: MitoTracker® Red CMXRos, a mitochondria marker.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636

GTX632636 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [GT20710] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632636

GTX632636 ICC/IF Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636

GTX632636 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

Anti-SDHA antibody [GT20710] used in Western Blot (WB). GTX632636

GTX632636 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

Anti-SDHA antibody [GT20710] used in IHC (Paraffin sections) (IHC-P). GTX632636

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Adriaenssens E et al. Nat Cell Biol 2023; 25 (3) : 467-480 Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.
实验应用 : WB
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