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SARS-CoV Spike overexpression 293T whole 细胞裂解物

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668
SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

目录号 GTX535668

实验应用

 WB

Speciess

 SARS Coronavirus
产品包装
200 μg (¥3500)
查看所有 SARS-CoV Spike 的产品
产品说明

摘要

Whole-cell lysate of 293T cells overexpressing SARS-CoV spike protein (full-length, no tag). The translated amino acid sequence is based on AAP13567.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

产品属性

形式

Liquid

存储溶液

50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A

保存剂

0.025% ProClin 300

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

GTX535668 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 1509661

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

GTX535668 WB Image

SARS-CoV spike 293T overexpression cell lysates (GTX535668) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 1509661

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SDS
Proclin.pdf
产品包装 报价 (¥)
¥ 3500
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