GeneTex中国官方网站
  • China

RFP抗体

Orthogonal
Anti-RFP antibody used in Immunoprecipitation (IP). GTX127897
Anti-RFP antibody used in Western Blot (WB). GTX127897
Anti-RFP antibody used in Western Blot (WB). GTX127897
Anti-RFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127897

目录号 GTX127897

文献引用 8

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IP

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RFP 的产品
产品说明

摘要

RFP antibody recognizes the red fluorescent protein (RFP), a fluorophore often used in conjunction with the green fluorescent protein (GFP). Like GFP, RFP allows visualization of fusion proteins in cells and is one of the most frequently used tools to monitor in vivo biological processes. This RFP antibody does not cross-react with the GFP protein.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:5000
IP 1:10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

阳性对照

293T expressing RFP-tagged protein

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Full length RFP recombinant protein

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885652

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Red fluorescent protein

研究领域

產品图片

Anti-RFP antibody used in Immunoprecipitation (IP). GTX127897

GTX127897 IP Image

RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody (GTX127897). (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detected by GTX127897 diluted at 1:10000.

Anti-RFP antibody used in Western Blot (WB). GTX127897

GTX127897 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (GTX127897) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RFP antibody used in Western Blot (WB). GTX127897

GTX127897 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (GTX127897) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127897

GTX127897 ICC/IF Image

RFP antibody detects RFP protein at by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFP protein stained by RFP antibody (GTX127897) diluted at 1:5000.
Red: RFP is expressed in the RFP-fusion protein tranfected cell.
Blue: Hoechst 33342 staining.
The cell of merger panel with no RFP expressed as a negative control.

文献引用

Application Reference

Syed Nooruzuha Barmaver et al. Research Square 2023; A Sideroexin/Connexin adaptor ties kinesin-3 to mitochondria in neurons
实验应用 : WB, IP
Jing Wu et al. Function (Oxf) 2022; 3 (3) : zqac017 Endothelial Cullin3 Mutation Impairs Nitric Oxide-Mediated Vasodilation and Promotes Salt-Induced Hypertension.
实验应用 : WB
Shi Fang et al. JCI Insight 2022; 7 (9) RhoBTB1 reverses established arterial stiffness in angiotensin II-induced hypertension by promoting actin depolymerization.
实验应用 : WB
Izumi Taku et al. Front Cell Infect Microbiol 2020; 10 : 610200 Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase 2 From the Endoplasmic Reticulum in Plasmodium falciparum.
实验应用 : IP
Mukohda M et al. J Clin Invest 2019; 129 (6) : 2318-2332 RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity.
显示更多 +
我要回报文献

产品评论

目前没有使用 RFP antibody (GTX127897) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com