GeneTex中国官方网站
  • China

Porcine circovirus type 2 / PCV2 replicase抗体

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638

目录号 GTX133638

文献引用 2
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Porcine circovirus type 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Porcine circovirus type 2 replicase 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

36 kDa. ( Note )

阳性对照

PCV2-infected PK-15

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Porcine circovirus type 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Porcine circovirus type 2 / PCV2 replicase (PCV2 virus (strain AUT1)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887057

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Porcine circovirus Replicase , Porcine circovirus Rep , PCV Replicase , PCV Rep

研究领域

產品图片

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638

GTX133638 ICC/IF Image

Porcine circovirus type 2 / PCV2 replicase antibody detects Porcine circovirus type 2 / PCV2 replicase protein by immunofluorescent analysis.
Sample: Uninfected and infected PK-15 cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: Porcine circovirus type 2 / PCV2 replicase stained by Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638) diluted at 1:50.
Image courtesy of Dr. Karuppannan from Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638

GTX133638 WB Image

Non-infected (–) and infected (+) PK-15 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Ouyang Y et al. Vet Res 2024; 55 (1) : 22 Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Pi?train pigs.
Zhang S et al. Molecules 2019; 24 (23) Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification.
我要回报文献

产品评论

Porcine circovirus type 2 / PCV2 replicase antibody 目录号 GTX133638

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638
Date : Anonymous submitted on 24-Sep-2019
Score :
Application Tested : WB
Sample Species : PCV2
Sample : PAM cells
Amount used : 30 μg
Blocking : 5 % non-fat milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638
Date : Anonymous submitted on 22-Jun-2018
Score :
Application Tested : ICC/IF
Sample Species : PCV2
Sample : PK-15 cells
Blocking : 1% BSA in PBS with 0.05% Tween, 37°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS, pH7.4, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.2% TritonX-100 in PBS, 25°C, 40 minHr
Primary Antibody : 1:50, 37°C, 1Hr
Figure Note : PCV2 non-infected (upper left) and infected (upper right, lower left and lower right) PK-15 cells. Image courtesy of Dr. Karuppannan from the lab "Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA".
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com