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Antigen retrieval condition-Pepsin (ready to use)

目录号 GTX30935

文献引用 1

实验应用

 IHC-P
产品包装
100 ml (¥2400)
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产品说明

摘要

Pepsin Antigen Retrieval Reagent (ready to use)

产品应用

应用说明

Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). Discard Pepsin reagent, wash 3-5 times with buffer.

产品属性

形式

Liquid

存放说明

Store at 2-8ºC, DO NOT FREEZE.

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

研究领域

文献引用

Application Reference

Ariel Diaz et al. J Cereb Blood Flow Metab 2021; 41 (9) : 2381-2394 Urokinase-type plasminogen activator promotes N-cadherin-mediated synaptic recovery in the ischemic brain.
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产品包装 报价 (¥)
¥ 2400
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