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PGP9.5抗体

KO/KD Orthogonal
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

目录号 GTX109637

文献引用 13
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 PGP9.5 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

25 kDa. ( Note )

阳性对照

Neuro2A , GL261 , rat brain , mouse brain

预测种属反应

Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952497

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1

细胞定位

Cytoplasm

功能与背景

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637

GTX109637 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109637

GTX109637 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody (GTX109637) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse skin.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:10000.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109637

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:5000

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PGP9.5 antibody (GTX109637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109637

GTX109637 IHC-P Image

The data was published in the journal Cells in 2020. PMID: 32155948

文献引用

Application Reference

Clayton DR et al. Am J Physiol Renal Physiol 2022; 323 (3) : F299-F321 Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts.
Chang WY et al. Lab Invest 2022; 102 (9) : 1023-1037 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.
实验应用 : IHC-Fr
种属反应 : Human, Mouse
Fede C et al. Sci Rep 2021; 11 (1) : 12623 Evidence of a new hidden neural network into deep fasciae.
实验应用 : IHC-P
种属反应 : Mouse
Koshimizu H et al. Life Sci 2020; 263 : 118577 Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model.
实验应用 : ICC/IF
种属反应 : Rat
Hong J et al. Neurosci Lett 2020; 737 : 135320 Identification of Cardiac Expression Pattern of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Receptor using a Transgenic Reporter Mouse Model.
实验应用 : IHC-Fr
种属反应 : Mouse
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产品评论

PGP9.5 antibody 目录号 GTX109637

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109637
Date : Anonymous submitted on 01-Aug-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Dorsal root ganglion
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C
Primary Antibody : 1:500, 4°C, 6Hr
Figure Note : PGP9.5 (left panel), and counterstain with hoechst (right panel).
我要评论产品
Application Reference
Clayton DR et al. Am J Physiol Renal Physiol 2022; 323 (3):F299-F321 Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts.
Chang WY et al. Lab Invest 2022; 102 (9):1023-1037 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.
实验应用 : IHC-Fr
种属反应 : Human, Mouse
Fede C et al. Sci Rep 2021; 11 (1):12623 Evidence of a new hidden neural network into deep fasciae.
实验应用 : IHC-P
种属反应 : Mouse
Koshimizu H et al. Life Sci 2020; 263:118577 Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model.
实验应用 : ICC/IF
种属反应 : Rat
Hong J et al. Neurosci Lett 2020; 737:135320 Identification of Cardiac Expression Pattern of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Receptor using a Transgenic Reporter Mouse Model.
实验应用 : IHC-Fr
种属反应 : Mouse
Canta A et al. Antioxidants (Basel) 2020; 9 (7)
实验应用 : IHC-P
种属反应 : Mouse
Dragunas G et al. FASEB J 2020; 34 (6):7703-7717 Cholinergic neuroplasticity in asthma driven by TrkB signaling.
实验应用 : IHC-Fr
种属反应 : Mouse
Pagella P et al. Cells 2020; 9 (3) Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons.
实验应用 : IHC-P
种属反应 : Human
Alberti P et al. Neuropharmacology 2020; 164:107905
实验应用 : IHC-P
种属反应 : Rat
Xu YM et al. Toxicol Res (Camb) 2019; 8 (6):994-1001 Cadmium telluride quantum dot-exposed human bronchial epithelial cells: a further study of the cellular response by proteomics.
Meregalli C et al. J Neuroinflammation 2018; 15 (1):232 high-dose intravenous immunoglobulins reduce nerve macrophage infiltration and the severity of *-induced peripheral neurotoxicity in rats.
实验应用 : IHC-P
种属反应 : Rat
Tu NH et al. Eur J Neurosci 2017; Na+ /K+ -ATPase coupled to endothelin receptor type B stimulates peripheral nerve regeneration via lactate signaling.
实验应用 : ICC/IF
种属反应 : Mouse
Kishi C et al. Biol Res Nurs 2015; 17 (2):135-41 Hypo-osmotic shock-induced subclinical inflammation of skin in a rat model of disrupted skin barrier function.
实验应用 : IHC-Fr
种属反应 : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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