产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
MCF-7 , MDA-MB-231 , DDDDK-tagged LEP-transfected 293T
预测种属反应
Rat, Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human Leptin Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885271
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
leptin , LEPD , OB , OBS
细胞定位
Secreted
功能与背景
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX109204 IHC-P Image
Leptin antibody detects Leptin protein at secreted on human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
Leptin antibody (GTX109204) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109204 IHC-P Image
Leptin antibody detects Leptin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse adrenal gland.Leptin stained by Leptin antibody (GTX109204) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109204 WB Image
Leptin antibody detects Leptin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
12 % SDS-PAGE
Leptin antibody (GTX109204) dilution: 1:1000
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GTX109204 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109204 WB Image
MCF-7 whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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