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Lactate Dehydrogenase抗体

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101
Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101
Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101
Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

目录号 GTX22101

宿主

Goat

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IP, ELISA

种属反应

Human, Rabbit
产品包装
1 mg (¥6600)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:5000
ICC/IF 1:200
IP 1:100
ELISA 1:2000-1:10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

10 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Rabbit

免疫原

Full length lactate dehydrogenase protein isolated from rabbit muscle.

纯化方式

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lactate dehydrogenase A , LDH-M

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step
of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase
family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding
different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of
this gene. (provided by RefSeq)

数据库

研究领域

產品图片

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

GTX22101 WB Image

Western blot analysis of anti-Lactate Dehydrogenase antibody (1:1000 dilution) in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). A single band of ~36 kDa corresponds to the expected molecular weight for the protein. Personal communication Kuldeep Patel.

Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101

GTX22101 ICC/IF Image

GeneTex biotinylated anti-lactate dehydrogenase antibody was used to stain MeOH/Acetone-fixed HeLa cells by immunofluorescence.

Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101

GTX22101 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX22101 Lactate Dehydrogenase antibody.
Dilution : 1:200

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

GTX22101 WB Image

WB analysis of HeLa whole cell lysate using GTX22101 Lactate Dehydrogenase antibody.
Lane 1-4 : Cytoplasmic extracts (CF)
Lane 5-8 : Nuclear extracts (NF)
Loading : 30 μg
Dilution : 1:400

文献引用

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SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 6600
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