GeneTex中国官方网站
  • China

LAMP1抗体 [GT25212]

KO/KD Comparison Orthogonal
Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

目录号 GTX634336

文献引用 5

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT25212

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-Fr

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 LAMP1 的产品
产品说明

摘要

LAMP1 antibody detects lysosome-associated membrane protein 1 (LAMP-1, or CD107a), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP1 and LAMP2 are major protein components of lysosomal membranes, though both proteins appear to have a more dynamic distribution that includes endosomes, autolysosomes, and multivesicular bodies. Nevertheless, LAMP1 is often used as an indicator of lysosomes in cells.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

HeLa,HeLa membrane extract

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human LAMP1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888439

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lysosomal associated membrane protein 1 , CD107a , LAMPA , LGP120

细胞定位

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

功能与背景

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336

GTX634336 ICC/IF Image

LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

KO/KD

Wild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Comparison Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Lin NY et al. Oncogenesis 2022; 11 (1) : 8 C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
实验应用 : ICC/IF
Ryskalin L et al. Nutrients 2021; 13 (10) Lactoferrin Protects against Methamphetamine Toxicity by Modulating Autophagy and Mitochondrial Status.
实验应用 : ICC/IF
Lin DS et al. Cells 2020; 9 (5) Impairment of Proteasome and Autophagy Underlying the Pathogenesis of Leukodystrophy.
实验应用 : IHC-Fr
Fararjeh AS et al. Cancers (Basel) 2019; 11 (4) Proteasome 26S Subunit, non-ATPase 3 (PSMD3) Regulates Breast Cancer by Stabilizing HER2 from Degradation.
实验应用 : WB
种属反应 : Human
Zhang Y et al. Nat Commun 2018; 9 (1) : 4176 Long non-coding RNA CCRR controls cardiac conduction via regulating intercellular coupling.
实验应用 : ICC/IF
我要回报文献

产品评论

目前没有使用 LAMP1 antibody [GT25212] (GTX634336) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com