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Jurkat whole 细胞裂解物

Jurkat whole cell lysate. GTX30596
Jurkat whole cell lysate. GTX30596

目录号 GTX30596

Speciess

 Human
产品包装
150 μl (¥3200)
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产品说明

摘要

Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na₃VO₄ were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

产品应用

阳性对照

Western analysis positive control. Note that Jurkat is only applicable as a positive control for specific antibodies.

产品属性

形式

Liquid

存储溶液

1x SDS sample buffer, 5% β-mercaptoethanol

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

產品图片

Jurkat whole cell lysate. GTX30596

GTX30596 Image

Jurkat whole cell lysate. GTX30596

GTX30596 Image

SDS-PAGE analysis of various cell lysates.
Lane 1 : HeLa cell lysate
Lane 2 : GTX30596
Lane 3 : 293T cell lysate
Lane 4 : MCF-7 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : Raji cell lysate
Loading : 20 μg

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产品包装 报价 (¥)
¥ 3200
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