Influenza B virus Nucleoprotein抗体

目录号 GTX128538

文献引用 4

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, ELISA, Sandwich ELISA
种属反应	Influenza B virus
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Influenza B virus Nucleoprotein 的产品

View product citations for antibody GTX128538 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:2000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629882 / GTX629883, Detection : GTX128538

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

62 kDa. ( Note )

阳性对照

Influenza B virus (Taiwan B70555) infected medium

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza B virus

免疫原

Recombinant fragment corresponding to N-terminal region of Influenza B virus NP (B/Taiwan/753/2005). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885796

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

Influenza B Virus Nucleoprotein , Influenza B Virus NP

研究领域

產品图片

GTX128538 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: Influenza B Virus infected MDCK cells were fixed in paraformaldehyde.
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128538) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX128538 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody (GTX128538) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 WB Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by Western blot analysis.
A. 5μl non-infected medium
B. 5μl Influenza B virus (Taiwan B70555) infected medium
7.5 % SDS-PAGE
Influenza B Virus NP antibody (GTX128538) dilution: 1:10000

GTX128538 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: MDCK cells infected with Influenza B virus (Taiwan B70555).
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128538) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128538 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

文献引用

Application Reference

Na L et al. Sci Adv 2024; 10 (5) : eadj4163 Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals.

Gravel C et al. iScience 2021; 24 (11) : 103328 Synthetic vaccine affords full protection to mice against lethal challenge of influenza B virus of both genetic lineages.

实验应用 : ELISA

Zhang Z et al. PLoS Pathog 2020; 16 (10) : e1008989 Selective usage of ANP32 proteins by influenza B virus polymerase: Implications in determination of host range.

实验应用 : WB

Jang Y et al. Antiviral Res 2016; 134 : 77-88 Antiviral activity of KR-23502 targeting nuclear export of influenza B virus ribonucleoproteins.

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Application Reference

Na L et al. Sci Adv 2024; 10 (5):eadj4163 Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals.

Gravel C et al. iScience 2021; 24 (11):103328 Synthetic vaccine affords full protection to mice against lethal challenge of influenza B virus of both genetic lineages.

实验应用 : ELISA

Zhang Z et al. PLoS Pathog 2020; 16 (10):e1008989 Selective usage of ANP32 proteins by influenza B virus polymerase: Implications in determination of host range.

实验应用 : WB

Jang Y et al. Antiviral Res 2016; 134:77-88 Antiviral activity of KR-23502 targeting nuclear export of influenza B virus ribonucleoproteins.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Influenza B virus Nucleoprotein抗体

目录号 GTX128538

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX128538 ICC/IF Image

GTX128538 WB Image

GTX128538 ELISA Image

GTX128538 ELISA Image

GTX128538 WB Image

GTX128538 ICC/IF Image

GTX128538 ELISA Image

GTX128538 ELISA Image

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