产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
rat brain , Mouse brain , 293T , A431 , HeLa , HepG2
预测种属反应
Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950529
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13
细胞定位
Mitochondrion matrix
功能与背景
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 ICC/IF Image
HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp60A is the homolog of mammalian Hsp60. (Gene ID: 32045/ UniProt: O02649)
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GTX110089 WB Image
HSP60 antibody detects HSPD1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
HSP60 antibody (GTX110089) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110089 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
The data was published in the journal J Biomed Sci in 2013. PMID: 24359494
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GTX110089 WB Image
The data was published in the journal Sci Rep in 2018. PMID: 29950685
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GTX110089 WB Image
The data was published in the journal Sci Rep in 2016. PMID: 27089984
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GTX110089 WB Image
The data was published in the journal Sci Rep in 2016. PMID: 27089984
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GTX110089 WB Image
The data was published in the journal Brain in 2014. PMID: 24727571
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文献引用
产品评论
HSP60 antibody
目录号 GTX110089
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Rating
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( Average 4.8 based on 4 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4.8 based on 4 users reviews)
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Date :
Anonymous submitted on 18-Apr-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells, H1975 cells
Amount used :
30 μg
Blocking :
5% BSA, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 08-Jan-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
PANC1 cells
Amount used :
25 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 23-Aug-2016
Application Tested :
WB
Sample Species :
Hm
Sample :
CHO DP 12 clone#1933 cells
Amount used :
40μg
Blocking :
3% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 13-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
W12 cells
Blocking :
1% skim milk in PBST, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
条带说明 :
Lane 1: W12 total cell lysate, Lane 2: W12 cytosol extract, Lane 3: W12 mitochondrial extract
