HMGB1抗体

Name: HMGB1抗体
Brand: GeneTex
SKU: GTX101277
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277

目录号 GTX101277

文献引用 22

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Wm, IHC, IHC (Free Floating)
种属反应	Human, Mouse, Rat, Zebrafish, Pig
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 HMGB1 的产品

View product citations for antibody GTX101277 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent
IHC	Assay dependent
IHC (Free Floating)	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

25 kDa. ( Note )

阳性对照

NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T

预测种属反应

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950499

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

细胞定位

Nucleus

功能与背景

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

数据库

Gene ID: 3146 HMGB1
UniProt: P09429 HMGB1

研究领域

產品图片

GTX101277 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101277 WB Image

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

GTX101277 IHC-Wm Image

HMGB1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by HMGB1 antibody (GTX101277) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

GTX101277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HMGB1 antibody (GTX101277) at 1:300 dilution.

GTX101277 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

GTX101277 WB Image

The data was published in the 2016 in Nat Commun. PMID: 27329339

GTX101277 ICC/IF Image

The data was published in the journal Oncotarget in 2018. PMID: 30279967

文献引用

Application Reference

Wen J et al. Int Immunopharmacol 2023; 119 : 110259 Knockdown of HMGB1 inhibits the crosstalk between oral squamous cell carcinoma cells and tumor-associated macrophages

实验应用 : WB

种属反应 : Human

Zhengke Li et al. FASEB J 2021; 35 (5) : e21373 ATR prevents Ca²⁺ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.

实验应用 : WB

种属反应 : Human

Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.

实验应用 : WB

种属反应 : Human

Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.

Kanno Y et al. Arthritis Res Ther 2020; 22 (1) : 76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.

实验应用 : WB

种属反应 : Mouse

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产品评论

HMGB1 antibody 目录号 GTX101277

Rating

( Average 5 based on 1 users reviews)

实验应用

IHC (Free Floating)(IHC (Free Floating))

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 14-Feb-2023

Score :

Application Tested : IHC (Free Floating)

Sample Species : Ms

Sample : adult mouse cerebral cortex tissue section

Blocking : 1% BSA in PBS, 25°C, 0.5Hr

Fixation : 4% PFA in PBS

Permeabilization /Antigen retrieval : 0.3% Triton X-100 / No antigen retireval

Primary Antibody : 1:500, 16°C, 4Hr

我要评论产品

Application Reference

Wen J et al. Int Immunopharmacol 2023; 119:110259 Knockdown of HMGB1 inhibits the crosstalk between oral squamous cell carcinoma cells and tumor-associated macrophages

实验应用 : WB

种属反应 : Human

Zhengke Li et al. FASEB J 2021; 35 (5):e21373 ATR prevents Ca²⁺ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.

实验应用 : WB

种属反应 : Human

实验应用 : WB

种属反应 : Human

Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.

Kanno Y et al. Arthritis Res Ther 2020; 22 (1):76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.

实验应用 : WB

种属反应 : Mouse

Huang L et al. Aging (Albany NY) 2019; 11 (17):6674-6690 RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways.

D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17):E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.

Contis-Montes de Oca A et al. Oncotarget 2018; 9 (70):33368-33381 N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a Histone Deacetylase Inhibitor, Induces the Release of Nuclear HMGB1 and Modifies ROS Levels in HeLa Cells.

Coleman LG Jr et al. Brain Behav Immun 2017; (Epub) HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism.

实验应用 : WB

种属反应 : Human

Masola V et al. FASEB J 2017; (Epub) Heparanase regulates the * polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.

实验应用 : ICC/IF

种属反应 : Human

Liu PL et al. PLoS One 2017; 12 (7):e0180844 MicroRNA-200c inhibits epithelial-mesenchymal transition, invasion, and migration of lung cancer by targeting HMGB1.

实验应用 : WB, ICC/IF, IHC-P

种属反应 : Human

Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.

实验应用 : WB

种属反应 : Mouse

Lin CY et al. Cell Death Discov 2016; 2:16065 Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.

实验应用 : WB

种属反应 : Human

Dick MS et al. Nat Commun 2016; 7:11929 ASC filament formation serves as a signal amplification mechanism for inflammasomes.

实验应用 : WB

种属反应 : Mouse

Bhatia A et al. Mol Ther Methods Clin Dev 2016; 3:16041 Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs.

实验应用 : IHC-P

种属反应 : Pig

Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.

实验应用 : ICC/IF

种属反应 : Human

Lin TJ et al. Mol Cancer 2015; 14:174 Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.

实验应用 : WB

种属反应 : Mouse

Juranek JK et al. Front Cell Neurosci 2015; 9:485 Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis.

实验应用 : WB

种属反应 : Human

Ruhl S et al. Eur J Immunol 2015; 45 (10):2927-36 Caspase-11 activates a canonical NLRP3 inflammasome by promoting K(+) efflux.

实验应用 : WB

种属反应 : Human

Xu YF et al. World J Gastroenterol 2015; 21 (11):3256-65 High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma.

实验应用 : IHC

种属反应 : Human

Lee SM et al. Int J Cardiol 2015; 190:353-9 Amitriptyline pharmacologically preconditions rat hearts against cardiac ischemic-reperfusion injury.

实验应用 : WB

种属反应 : Rat

Kayagaki N et al. Nature 2011; 479 (7371):117-21 Non-canonical inflammasome activation targets caspase-11.

实验应用 : WB

种属反应 : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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HMGB1抗体

目录号 GTX101277

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX101277 WB Image

GTX101277 IHC-P Image

GTX101277 IHC-P Image

GTX101277 IHC-P Image

GTX101277 IHC-P Image

GTX101277 IHC-P Image

GTX101277 IHC-P Image

GTX101277 WB Image

GTX101277 WB Image

GTX101277 WB Image

GTX101277 WB Image

GTX101277 WB Image

GTX101277 ICC/IF Image

GTX101277 IHC-P Image

GTX101277 ICC/IF Image

GTX101277 ICC/IF Image

GTX101277 IHC-Wm Image

GTX101277 IHC-P Image

GTX101277 WB Image

GTX101277 WB Image

GTX101277 ICC/IF Image

文献引用

Application Reference

产品评论

HMGB1 antibody 目录号 GTX101277

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