HA tag抗体 [HL1603]

Name: HA tag抗体 [HL1603]
Brand: GeneTex
SKU: GTX637082
Price: 4000.00 RMB
Rating: 4 (1 reviews)

Anti-HA tag antibody [HL1603] used in Western Blot (WB). GTX637082

Anti-HA tag antibody [HL1603] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637082

Anti-HA tag antibody [HL1603] used in ELISA (ELISA). GTX637082

Anti-HA tag antibody [HL1603] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637082

目录号 GTX637082

宿主	Rabbit
克隆	Monoclonal
克隆编号	HL1603
同种型	IgG
实验应用	WB, ICC/IF, ELISA, IHC-P (cell pellet)
种属反应	Species independent
产品包装	100 μl (¥4000), 25 μl (¥1700)

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说明书

View product citations for antibody GTX637082 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	Assay dependent
ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to HA tag.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX637082 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with HA tag antibody [HL1603] (GTX637082) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX637082 ICC/IF Image

HA tag antibody [HL1603] detects HA tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA tag stained by HA tag antibody [HL1603] (GTX637082) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637082 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (GTX637082) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX637082 IHC-P (cell pellet) Image

HA tag antibody [HL1603] detects HA tag protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and HA tag - transfected 293T cell pellet.
Green: HA tag stained by HA tag antibody [HL1603] (GTX637082) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

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产品评论

HA tag antibody [HL1603] 目录号 GTX637082

Rating

( Average 4 based on 1 users reviews)

实验应用

Western Blot(WB)

( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 18-Apr-2023

Score :

Application Tested : WB

Sample : TurboID-6HA

Amount used : 400ug

Blocking : 3% skim milk in TBS, 28°C, 0.5Hr

Primary Antibody : 1:2000, 4°C, 16Hr

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产品包装

报价 (¥)

100 μl

¥ 4000

25 μl

¥ 1700

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