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HA tag抗体 [GT4810]

Comparison
Anti-HA tag antibody [GT4810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628902
Anti-HA tag antibody [GT4810] used in Immunoprecipitation (IP). GTX628902
Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902
Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

目录号 GTX628902

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT4810

同种型

 IgG2a

实验应用

 WB, ICC/IF, IP

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 HA tag 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

HeLa cell expressing HA-tagged protein

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to HA tag

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888078

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

HA tag antibody , HA antibody , HA epitope tag antibody

研究领域

產品图片

Anti-HA tag antibody [GT4810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628902

GTX628902 ICC/IF Image

HA tag antibody [GT4810] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT4810] (GTX628902) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-HA tag antibody [GT4810] used in Immunoprecipitation (IP). GTX628902

GTX628902 IP Image

HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody (GTX628902) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] (GTX628902) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

GTX628902 WB Image

HA tag antibody [GT4810] detects HA tag protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA tagged protein expressing HeLa cells
5% SDS-PAGE
HA tag antibody [GT4810] (GTX628902) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

GTX628902 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT4810] (GTX628902) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

文献引用

Application Reference

Ramanantsalama MR et al. J Cell Sci 2022; 135 (11) : jcs259893 TFK1, a basal body transition fibre protein that is essential for cytokinesis in Trypanosoma brucei
实验应用 : ICC/IF
Charlotte Isch et al. PLoS Pathog 2021; 17 (8) : e1009329 Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar.
实验应用 : ICC/IF
Isch C. et al. bioRxiv 2021; (Epub) Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar.
实验应用 : ICC/IF
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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