宿主 |
Goat |
|---|---|
克隆 |
Polyclonal |
同种型 |
IgG |
实验应用 |
WB, IP, ELISA, IHC |
种属反应 |
Species independent |
| 产品包装 |
100 μg (¥4300)
|
| 实验应用 | 稀释倍数 |
|---|---|
| WB | 1:1000-1:10000 |
| IP | Assay dependent |
| ELISA | 1:20000-1:50000 |
| IHC | 1:1:1000-1:10000 |
|
GTX26633 WB ImageGeneTex anti-GST polyclonal antibody (GTX26633) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 μg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4º C. For detection DyLight™800 conjugated Donkey-a-Goat IgG was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25º C. |
|
GTX26633 IP ImageImmunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 μg per lane. Primary antibody: anti-GST 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. |
|
GTX26633 ELISA Image
ELISA analysis of recombinant GST protein using serially diluted GTX26633 GST tag antibody. |
|
GTX26633 WB Image
WB analysis of various samples using GTX26633 GST tag antibody. |
|
GTX26633 WB Image
WB analysis of recombinant GST proteins using GTX26633 GST tag antibody. |
|
GTX26633 IP Image
IP analysis of recombinant proteins using GTX26633 GST tag antibody. |
| SDS | |
|---|---|
| Sodium Azide.pdf | |
| 产品包装 | 报价 (¥) |
|---|---|
| ¥ 4300 | |
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| http://www.neobioscience.com | |
| genetex@neobioscience.com |
