GST tag抗体 [8-826]

目录号 GTX79438

宿主	Mouse
克隆	Monoclonal
克隆编号	8-826
同种型	IgG
实验应用	WB, ICC/IF, IP, ELISA
种属反应	Species independent
产品包装	100 μl (¥5300)

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说明书
文件

View product citations for antibody GTX79438 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000
ICC/IF	Assay dependent
IP	2 μg
ELISA	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 0.1% BSA

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant purified full-length GST

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_11178764

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX79438 IP Image

Immuno-precipitation of GST-Shp2 SH2 domain (Lane 2, 39kDa) was performed by mixing 2μg of purified fusion protein with 1μg of GST antibody [8-326] overnight on a rocking platform at 4ºC. The immune-complex was then captured on 50μl Protein A/G Agarose. Captured immune-complexes were then washed extensively and proteins eluted. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. IP efficiency was compared to a non-IP loading control consisting of 1ug GST-Shp2 SH2 domain (Lane1). Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight rotating at 4ºC. Membranes were then washed in TBS/0.1% Tween-20 and probed with a proper secondary antibody. Membranes were washed and chemiluminescent detection was performed.

GTX79438 WB Image

Western blot analysis of GST-Abl SH2 domain (Lane 1, 35kDa) and GST-Grb2 SH2 domain (Lane 2, 38kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with GST antibody [8-326] at a dilution of 1:1000 overnight at 4ºC on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.

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SDS
PBS.pdf
Sodium Azide.pdf

产品包装

报价 (¥)

100 μl

¥ 5300

Qty

GST tag抗体 [8-826]

目录号 GTX79438

宿主

克隆

克隆编号

同种型

实验应用

种属反应