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GFP抗体

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617

目录号 GTX113617

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, Dot, ELISA, IHC

种属反应

Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
产品说明

摘要

GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:1000-1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IP 1:100-1:500
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

293T expressing GFP-tagged protein

产品说明

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Full length GFP recombinant protein

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950371

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Green Fluorescent Protein , GFP , eGFP , enhanced green fluorescent protein

研究领域

產品图片

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP(GTX113617) antibody at 1:200 dilution.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617

GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) diluted at 1:10000.

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg 293T whole cell extract expressing GFP-tagged protein
C. 1 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617

GTX113617 WB IP Image

The data was published in the 2020 in Front Mol Neurosci. PMID: 32587503

Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617

GTX113617 WB IP Image

The data was published in the journal J Virol in 2016. PMID: 27489281

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Adv Sci (Weinh) in 2020. PMID: 32195080

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617

GTX113617 IHC-P Image

The data was published in the journal EMBO Mol Med in 2014. PMID: 24448489

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575

Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617

GTX113617 WB IP Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal J Biomed Sci in 2018.PMID: 29361943

Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617

GTX113617 IHC-Fr Image

The data was published in the journal Nat Commun in 2018.PMID: 30310071

Anti-GFP antibody used in Western Blot (WB). GTX113617

GTX113617 WB Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

文献引用

产品评论

GFP antibody 目录号 GTX113617

Rating ( Average 4.9 based on 8 users reviews)
实验应用
Western Blot(WB) ( Average 4.8 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : WB
Sample : Transgenic mouse treated with TAM
Amount used : 20μg
Blocking : 5% gelatin in T-TBS, 20°C, 0.5Hr
Primary Antibody : 1:1500, 4°C, 16Hr
Figure Note : Intestinal specific GFP expression was examined in transgenic mouse treated with TAM.
Date : Anonymous submitted on 31-Aug-2021
Score :
Application Tested : IHC-P
Sample : mFLE-EGFP mouse testis, 2-dpp, paraffin-section 5μm
Blocking : 5% donkey-serum in TNB, 0.3% Triton X-100, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : The 2-dpp testis tissue section of mFLE-EGFP transgenic mouse which present fetal Leydig cell (fLC) was stained by GFP antibody and HSD-3b1 antibody. GFP only expressed in fLC.
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% nonfat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used : 15μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
条带说明 : A: U2OS cells transfected with GFP-p53, B: U2OS cells transfected with control plasmid (no GFP-tag)
Date : Anonymous submitted on 12-Apr-2012
Score :
Application Tested : WB
Sample : Protoplast from Rosette leaves
Amount used : 30μg
Blocking : 5% milk in PBST, 4°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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