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GFP抗体 (FITC)

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662
Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662
Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662
Anti-GFP antibody (FITC) used in Dot blot (Dot). GTX26662
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Anti-GFP antibody (FITC) used in IHC (Whole mount) (IHC-Wm). GTX26662

目录号 GTX26662

文献引用 17

宿主

 Goat

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-Fr, IHC-Wm, FACS, Dot, IHC, Multiplexing

种属反应

 Species independent
产品包装
100 μg (¥4300)
查看所有 GFP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB >1:10000
ICC/IF 1:500-1:2500
IHC-Fr Assay dependent
IHC-Wm Assay dependent
FACS Assay dependent
Dot Assay dependent
IHC Assay dependent
Multiplexing Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

No reaction was observed against Human, Mouse and Rat Serum Proteins.

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Jellyfish

免疫原

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

纯化方式

Purified by antigen-affinity chromatography.
From serum

偶联

Fluorescein isothiocyanate (FITC)
Ratio : 3.85 molecules FITC per Goat IgG molecule.

RRID

AB_371424

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

研究领域

產品图片

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662

GTX26662 WB Image

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.

Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662

GTX26662 IHC-Fr Image

Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662

GTX26662 WB Image

WB analysis of recombinant GFP protein using GTX26662 GFP antibody (FITC).
Loading : 50 ng
Dilution : 1:1000

Anti-GFP antibody (FITC) used in Dot blot (Dot). GTX26662

GTX26662 Dot Image

Dot blot analysis of GFP using GTX26662 GFP antibody (FITC).
Loading : Five point 3-fold serial dilution starting at 100 ng (Dot 1).
Dilution : 1:1000

Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662

GTX26662 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26960923

Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662

GTX26662 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26960923

Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662

GTX26662 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26960923

Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662

GTX26662 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26960923

Anti-GFP antibody (FITC) used in IHC (Whole mount) (IHC-Wm). GTX26662

GTX26662 IHC-Wm Image

The data was published in the journal PLoS Genet in 2008.PMID: 18404215

文献引用

Application Reference

Evsen L et al. Development 2020; 147 (15) let-7 miRNAs inhibit CHD7 expression and control auditory-sensory progenitor cell behavior in the developing inner ear.
Laiouar S et al. Curr Biol 2020; 30 (7) : 1177-1188.e5 RabX1 Organizes a Late Endosomal Compartment that Forms Tubular Connections to Lysosomes Consistent with a "Kiss and Run" Mechanism.
Lale Evsen et al. bioRxiv 2019; (Epub) Let-7 miRNAs control auditory sensory progenitor behavior in the vertebrate inner ear.
实验应用 : IHC-Fr
Clayton ZE et al. Biosci Rep 2018; (Epub) Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model.
实验应用 : IHC
Chan AH et al. PLoS One 2017; 12 (3) : e0174773 Evaluation of synthetic vascular grafts in a mouse carotid grafting model.
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Application Reference
Evsen L et al. Development 2020; 147 (15) let-7 miRNAs inhibit CHD7 expression and control auditory-sensory progenitor cell behavior in the developing inner ear.
Laiouar S et al. Curr Biol 2020; 30 (7):1177-1188.e5 RabX1 Organizes a Late Endosomal Compartment that Forms Tubular Connections to Lysosomes Consistent with a "Kiss and Run" Mechanism.
Lale Evsen et al. bioRxiv 2019; (Epub) Let-7 miRNAs control auditory sensory progenitor behavior in the vertebrate inner ear.
实验应用 : IHC-Fr
Clayton ZE et al. Biosci Rep 2018; (Epub) Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model.
实验应用 : IHC
Chan AH et al. PLoS One 2017; 12 (3):e0174773 Evaluation of synthetic vascular grafts in a mouse carotid grafting model.
Woichansky I et al. Nat Commun 2016; 7:10834 Three mechanisms control E-cadherin localization to the zonula adherens.
实验应用 : IHC
Raft S et al. Dev Biol 2014; 390 (1):51-67 Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear.
Raft S et al. PLoS One 2014; 9 (10):e109043 Pou3f4-mediated regulation of ephrin-b2 controls temporal bone development in the mouse.
实验应用 : ICC/IF
de Renty C et al. PLoS One 2014; 9 (5):e97434 Cytoplasmic Localization of p21 Protects Trophoblast Giant Cells from DNA Damage Induced Apoptosis.
实验应用 : IHC-Wm
Berns N et al. J Cell Sci 2014; A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche.
实验应用 : ICC/IF
Li X et al. PLoS Genet 2013; 9 (7):e1003641 SLC26A4 targeted to the endolymphatic sac rescues hearing and balance in Slc26a4 mutant mice.
Evsen L et al. J Neurosci 2013; 33 (9):3879-90 Progression of neurogenesis in the inner ear requires inhibition of Sox2 transcription by neurogenin1 and neurod1.
实验应用 : IHC
Gomez JM et al. J Cell Biol 2012; 199 (7):1131-43 Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis.
Klausner EA et al. J Gene Med 2012; 14 (2):100-8 Corneal gene delivery: chitosan oligomer as a carrier of CpG rich, CpG free or S/MAR plasmid DNA.
Mercapide J et al. Int J Cancer 2010; 126 (5):1206-15 Primary gene-engineered neural stem/progenitor cells demonstrate tumor-selective migration and antitumor effects in glioma.
Hwang CH et al. Dev Dyn 2009; 238 (11):2725-34 Foxg1 is required for proper separation and formation of sensory cristae during inner ear development.
实验应用 : IHC-Wm
Chang W et al. PLoS Genet 2008; 4 (4):e1000050 Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements.
SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 4300
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