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GFAP抗体

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

目录号 GTX108711

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, IHC

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

GFAP-transfected 293T

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037091

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glial fibrillary acidic protein , ALXDRD

细胞定位

Cytoplasm

功能与背景

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.
Blue: Hoechst 33342 staining.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

The data was published in the journal Cancer Med in 2019. PMID: 31274246

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32098078

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

The data was published in the 2022 in Sci Rep. PMID: 35027586

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

The data was published in the 2019 in Sci Rep. PMID: 30931957

文献引用

产品评论

GFAP antibody 目录号 GTX108711

Rating ( Average 5 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : S.D. rat brain sagittal cryo-section,50μm
Blocking : 1% BSA + 0.5% Troton X-100/PBS,pH=7.4, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
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