摘要
EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Sheep IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to sheep IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.
To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-sheep IgG (Optimized for Prot A/G) (GTX228911-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.
形式
Liquid
存储溶液
0.05M Tris, 0.15M NaCl, 1%BSA, 40% Glycerol
保存剂
0.005% Thimerosal
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Sheep
Target Isotype
IgG
纯化方式
Affinity purified by Protein G.
偶联
Horseradish peroxidase(HRP)
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
