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EasyBlot anti Rabbit IgG (HRP)

EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
EasyBlot anti Rabbit IgG (HRP). GTX221666-01

目录号 GTX221666-01

文献引用 52
1

宿主

 Mouse

克隆

 Monoclonal

同种型

 IgG1

实验应用

 WB

种属反应

 Rabbit
产品包装
250 μl (¥2300),
50 μl (¥1100)
查看所有 Rabbit IgG 的产品
产品说明

摘要

EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000

Note :

WB
Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

native-rabbit IgG

产品属性

形式

Liquid

存储溶液

0.05M Tris, 0.15M NaCl, 1%BSA, 40% Glycerol

保存剂

0.025% ProClin 300

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Rabbit

Target Isotype

IgG

纯化方式

Affinity purified by Protein G.

偶联

Horseradish peroxidase(HRP)

RRID

AB_10620421

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

產品图片

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).  The precipitated protein was detected by GTX102965 diluted at 1:10000.

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.

EasyBlot anti Rabbit IgG (HRP). GTX221666-01

GTX221666-01 WB Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

文献引用

Application Reference

YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus
F Ye et al. Cell Rep 2024; 43 (4) : 114002 Endocytic activation and exosomal secretion of matriptase stimulate the second wave of EGF signaling to promote skin and breast cancer invasion
HN Huang et al. Int J Mol Sci 2024; 25 (3) : 1374 Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Zelinger L et al. iScience 2023; 26 (4) : 106417 Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.
June Seok Heo et al. Sci Rep 2022; 12 (1) : 2776 Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.
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产品评论

EasyBlot anti Rabbit IgG (HRP) 目录号 GTX221666-01

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
EasyBlot anti Rabbit IgG (HRP). GTX221666-01
Date : Anonymous submitted on 02-Feb-2018
Score :
Application Tested : WB
Sample : HSC2 cells
条带说明 : Immunoprecipitation of Tel2 protein. Input, HSC2 cells (Lane 1). HSC2 cells subjected to immunoprecipitation using Tel2 antibody (Lane 2) or normal rabbit IgG (Lane 3). The precipitated protein was detected by RUVBL1 antibody. EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent in Western blot and can avoid the interference of IgG heavy chains and light chains.
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Application Reference
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F Ye et al. Cell Rep 2024; 43 (4):114002 Endocytic activation and exosomal secretion of matriptase stimulate the second wave of EGF signaling to promote skin and breast cancer invasion
HN Huang et al. Int J Mol Sci 2024; 25 (3):1374 Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Zelinger L et al. iScience 2023; 26 (4):106417 Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.
June Seok Heo et al. Sci Rep 2022; 12 (1):2776 Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 2300
¥ 1100
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