产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse cerebellum , rat cerebellum
预测种属反应
Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of mouse EAAT1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887194
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
solute carrier family 1 (glial high affinity glutamate transporter), member 3 , AI504299 , B430115D02Rik , Eaat1 , GLAST , GLAST-1 , GLU-T , GluT-1 , Gmt1 , MGluT1
细胞定位
Membrane
功能与背景
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
数据库
研究领域
產品图片
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GTX134059 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX134059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134059 IHC-P Image
EAAT1 antibody detects EAAT1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: EAAT1 stained by EAAT1 antibody (GTX134059) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134059 IHC-Fr Image
EAAT1 antibody detects EAAT1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: EAAT1 stained by EAAT1 antibody (GTX134059) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX134059 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX134059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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