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E-Cadherin抗体 [N3C2], Internal

KO/KD Comparison Orthogonal
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178
Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

目录号 GTX124178

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Rat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 E-Cadherin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

阳性对照

PC-12 , MCF-7

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human E-Cadherin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11168478

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

细胞定位

Cell junction , Cell membrane

功能与背景

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX124178

GTX124178 IP Image

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody [N3C2], Internal (GTX124178).
Western blot analysis was performed using E-Cadherin antibody [N3C2], Internal (GTX124178).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-E-Cadherin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX124178

GTX124178 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (GTX124178) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124178

GTX124178 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX124178) diluted at 1:1000.

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-E-Cadherin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124178

GTX124178 ICC/IF Image

E-Cadherin antibody [N3C2], Internal detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

E-Cadherin antibody [N3C2], Internal detects E-Cadherin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-E-Cadherin antibody [N3C2], Internal used in Western Blot (WB). GTX124178

GTX124178 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID:  20356845

文献引用

Application Reference

Luo SD et al. J Exp Clin Cancer Res 2022; 41 (1) : 215 Aberrant miR-874-3p/leptin/EGFR/c-Myc signaling contributes to nasopharyngeal carcinoma pathogenesis.
Ling-Yea Yu et al. Sci Adv 2021; 7 (31) Synthetic dysmobility screen unveils an integrated STK40-YAP-MAPK system driving cell migration.
Yen-Hao Chen et al. Cancers (Basel) 2021; 13 (16) CD73 Promotes Tumor Progression in Patients with Esophageal Squamous Cell Carcinoma.
实验应用 : WB
种属反应 : Human
Chen YH et al. BMC Cancer 2021; 21 (1) : 979 Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study.
实验应用 : WB
种属反应 : Human
Ling HH et al. Exp Cell Res 2016; Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
实验应用 : WB
种属反应 : Human
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