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E-Cadherin抗体 [GT477]

KO/KD Comparison Orthogonal
Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691
Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Flow cytometry (FACS). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Immunoprecipitation (IP). GTX629691
Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691

目录号 GTX629691

文献引用 4
2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT477

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P, FACS, IP

种属反应

 Human, Mouse, Rat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 E-Cadherin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

阳性对照

MCF-7

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888129

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

细胞定位

Cell junction , Cell membrane

功能与背景

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Flow cytometry (FACS). GTX629691

GTX629691 FACS Image

E-Cadherin antibody [GT477] (GTX629691) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT477] (GTX629691) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Immunoprecipitation (IP). GTX629691

GTX629691 IP Image

E-adherin antibody [GT477] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Mouse IgG
B. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT477] (GTX629691)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT477] (GTX629691) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX635000) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000.

文献引用

Application Reference

A Adamiok-Ostrowska et al. Oncol Lett 2023; Agrin is a novel oncogenic protein in thyroid cancer
T?nia Lima et al. Cancers (Basel) 2022; 14 (8) Application of Proteogenomics to Urine Analysis towards the Identification of Novel Biomarkers of Prostate Cancer: An Exploratory Study.
实验应用 : WB
Hongtao Luo et al. Front Public Health 2020; 8 : 579705 Carbon Ion Therapy Inhibits Esophageal Squamous Cell Carcinoma Metastasis by Upregulating STAT3 Through the JAK2/STAT3 Signaling Pathway.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
实验应用 : WB
种属反应 : Human
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产品评论

E-Cadherin antibody [GT477] 目录号 GTX629691

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691
Date : Hu Jing-Lan submitted on 07-May-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Breast cancer cell line
Fixation : 4% PFA in PBS, 25°C, 0.25Hr
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 25°C, 0.25Hr
Primary Antibody : Undiluted, 4°C, 24Hr
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : ca922 cells
Amount used : 20 μg
Blocking : 10% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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