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Dishevelled 3抗体 [N1N2], N-term

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Western Blot (WB). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX102509

目录号 GTX102509

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC

种属反应

 Human, Mouse, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

78 kDa. ( Note )

阳性对照

HCT116

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Dishevelled 3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240709

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

dishevelled segment polarity protein 3 , DRS3

细胞定位

Cytoplasm

功能与背景

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102509

GTX102509 IHC-P Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102509

GTX102509 ICC/IF Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dishevelled 3 protein stained by Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Dishevelled 3 antibody [N1N2], N-term used in Western Blot (WB). GTX102509

GTX102509 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 7.5% SDS PAGE
GTX102509 diluted at 1:10000

Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX102509

GTX102509 IHC Image

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled 3 antibody [N1N2], N-term (GTX102509) at a 1:200 dilution.

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX102509

GTX102509 IHC-Fr Image

The data was published in the journal PLoS One in 2013. PMID: 23437169

文献引用

Application Reference

Pai VC et al. Oncogene 2018; (Epub) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
实验应用 : WB, IP
种属反应 : Human
Wang J et al. Clin Cancer Res 2013; 19 (20) : 5580-90 Integrative genomics analysis identifies candidate drivers at 3q26-29 amplicon in squamous cell carcinoma of the lung.
Rodriguez-Gil DJ et al. PLoS One 2013; 8 (2) : e56561 Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
实验应用 : IHC
种属反应 : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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