GeneTex中国官方网站
  • China

Creatine kinase (muscle)抗体 [N2C3]

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunohistochemistry (IHC). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

目录号 GTX108969

文献引用 1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Wm, IHC

种属反应

 Human, Mouse, Zebrafish, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Creatine kinase (muscle) 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

43 kDa. ( Note )

阳性对照

HeLaS3 , 293T , A431

预测种属反应

Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949986

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

creatine kinase, M-type , CKMM , CPK-M , M-CK

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunohistochemistry (IHC). GTX108969

GTX108969 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:200 dilution.

Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX108969

GTX108969 IHC-Wm Image

Creatine kinase (muscle) antibody [N2C3] detects Ckma protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:100.
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

GTX108969 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:300 dilution.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108969

GTX108969 ICC/IF Image

Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX108969) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Creatine kinase (muscle) antibody detects Creatine kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted by 1:1000.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
GTX108969 diluted at 1:1000

Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

GTX108969 IHC-P Image

Creatine kinase (muscle) antibody [N2C3] detects Creatine kinase (muscle) protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Wakao J et al. Biochem Biophys Res Commun 2017; (Epub) Efficient direct conversion of human fibroblasts into myogenic lineage induced by co-transduction with MYCL and MYOD1.
实验应用 : IHC-P
种属反应 : Mouse
我要回报文献

产品评论

目前没有使用 Creatine kinase (muscle) antibody [N2C3] (GTX108969) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com