产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A: 293T , B: HepG2 , C: A375 , A: mouse cerebellum
预测种属反应
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cav beta 4. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949796
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
calcium voltage-gated channel auxiliary subunit beta 4 , CAB4 , CACNLB4 , EA5 , EIG9 , EJM , EJM4 , EJM6
功能与背景
This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202](/templates/default/js/lightbox/images/loading.gif "Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202")
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GTX100202 ICC/IF Image
CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term (GTX100202) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX100202 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
C: A375
7.5% SDS PAGE
GTX100202 diluted at 1:5000
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GTX100202 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4(GTX100202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100202 WB Image
Sample (50 ug of whole cell lysate)
A: mouse cerebellum
7.5% SDS PAGE
GTX100202 diluted at 1:1000
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GTX100202 IHC-Fr Image
Cav beta 4 antibody [C3], C-term detects Cav beta 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Cav beta 4 protein stained by Cav beta 4 antibody [C3], C-term (GTX100202) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202](/upload/website/prouct_img/small/GTX100202/GTX100202_39391_IFA_w_23053123_757.webp "Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202](/upload/website/prouct_img/small/GTX100202/GTX100202_39391_WB_w_23053123_298.webp "Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100202](/upload/website/prouct_img/small/GTX100202/GTX100202_39391_IHC_w_23053123_294.webp "Anti-Cav beta 4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202](/upload/website/prouct_img/small/GTX100202/GTX100202_39391_WB_M_cerebellum_w_23053123_705.webp "Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100202](/upload/website/prouct_img/small/GTX100202/GTX100202_39391_20170608_IHC-P_M_w_23053123_767.webp "Anti-Cav beta 4 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202](/upload/website/prouct_img/normal/GTX100202/GTX100202_39391_IFA_w_23053123_757.webp "Anti-Cav beta 4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202](/upload/website/prouct_img/normal/GTX100202/GTX100202_39391_WB_w_23053123_298.webp "Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100202](/upload/website/prouct_img/normal/GTX100202/GTX100202_39391_IHC_w_23053123_294.webp "Anti-Cav beta 4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202](/upload/website/prouct_img/normal/GTX100202/GTX100202_39391_WB_M_cerebellum_w_23053123_705.webp "Anti-Cav beta 4 antibody [C3], C-term used in Western Blot (WB). GTX100202")
![Anti-Cav beta 4 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100202](/upload/website/prouct_img/normal/GTX100202/GTX100202_39391_20170608_IHC-P_M_w_23053123_767.webp "Anti-Cav beta 4 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100202")
