Calnexin抗体 [C3], C-term

Name: Calnexin抗体 [C3], C-term
Brand: GeneTex
SKU: GTX109669
Price: 4000.00 RMB
Rating: 5 (3 reviews)

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

目录号 GTX109669

文献引用 31

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse, Rat, Sheep
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX109669 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T

产品说明

IP/MS validation was supported by references (PMID:30377396)

预测种属反应

Bovine, Dog, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949824

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

calnexin , CNX , IP90 , P90

细胞定位

Endoplasmic reticulum membrane , Melanosome

功能与背景

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

数据库

Gene ID: 821 CANX
UniProt: P27824 CANX

研究领域

產品图片

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 IP Image

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 WB Image

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

GTX109669 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 WB Image

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 WB Image

The data was published in the journal Skelet Muscle in 2014. PMID: 25530838

GTX109669 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 28615694

GTX109669 WB Image

The data was published in the journal Nat Commun in 2015.PMID: 25898173

GTX109669 WB Image

The data was published in the journal J Clin Med in 2019.PMID: 31167519

文献引用

Application Reference

Hsu XR et al. J Exp Clin Cancer Res 2023; 42 (1) : 283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.

XR Hsu et al. Journal of Experimental & Clinical Cancer Research 2023; 42 : 283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer

Kim M et al. J Biol Chem 2023; 299 (10) : 105242 Elexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics.

Zhao X et al. Front Cell Neurosci 2022; 16 : 1021592 Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.

Ge Gao et al. Am J Cancer Res 2022; 12 (5) : 1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

实验应用 : WB

种属反应 : Human

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我要回报文献

产品评论

Calnexin antibody [C3], C-term 目录号 GTX109669

Rating

( Average 5 based on 3 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 3 users reviews)

Date : Anonymous submitted on 06-Feb-2024

Score :

Application Tested : WB

Sample Species : Ms

Sample : RAW264.7 cells with/without Calnexin KO treatment

Primary Antibody : 1:1000, 4°C, >12Hr

Date : Anonymous submitted on 05-May-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : A549 cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST, 27°C, 1Hr

Primary Antibody : 1:5000, 4°C, 12Hr

Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr

条带说明 : Two groups (Dengue Nucleocapsid protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with calnexin antibody (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Date : Anonymous submitted on 30-Apr-2014

Score :

Application Tested : WB

Sample Species : Hu

Sample : HEK293 cells, KN73 cells

Amount used : 15μg

Blocking : Superblock blocking buffer in TBS, 24°C, 1Hr

Primary Antibody : 1:5000, 4°C, 16Hr

条带说明 : Lane A: HEK293 cells, Lane B: KN73 cells

我要评论产品

Application Reference

Hsu XR et al. J Exp Clin Cancer Res 2023; 42 (1):283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.

XR Hsu et al. Journal of Experimental & Clinical Cancer Research 2023; 42:283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer

Kim M et al. J Biol Chem 2023; 299 (10):105242 Elexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics.

Zhao X et al. Front Cell Neurosci 2022; 16:1021592 Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.

Ge Gao et al. Am J Cancer Res 2022; 12 (5):1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

实验应用 : WB

种属反应 : Human

Vultaggio-Poma V et al. Theranostics 2022; 12 (2):859-874 Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation.

实验应用 : WB

种属反应 : Human

Pegoraro A et al. Cell Death Dis 2021; 12 (12):1088 P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells.

实验应用 : WB

种属反应 : Mouse

Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.

实验应用 : WB

种属反应 : Mouse

Jonathan P Davies et al. ACS Infect Dis 2020; 6 (12):3174-3189 Comparative Multiplexed Interactomics of SARS-CoV-2 and Homologous Coronavirus Nonstructural Proteins Identifies Unique and Shared Host-Cell Dependencies.

实验应用 : WB, IP

种属反应 : Human

Madison T Wright et al. Mol Cell Proteomics 2020; 20:100008 Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

实验应用 : WB

种属反应 : Human

Hanif A et al. Oncotarget 2020; 11 (23):2246-2258 Exploring the role of survivin in neuroendocrine neoplasms.

Gaballa R et al. Cancers (Basel) 2020; 12 (8) Exosomes-Mediated Transfer of Itga2 Promotes Migration and Invasion of Prostate Cancer Cells by Inducing Epithelial-Mesenchymal Transition.

实验应用 : WB

种属反应 : Human

Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.

实验应用 : WB

种属反应 : Human

Jiang Y et al. J Cell Mol Med 2020; 24 (7):4356-4361 Novel mutations in ADAMTS13 CUB domains cause abnormal pre-mRNA splicing and defective secretion of ADAMTS13.

实验应用 : ICC/IF

Kuo HF et al. J Clin Med 2019; 8 (6) Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.

实验应用 : WB

种属反应 : Human, Rat

Inoue J et al. J Virol 2019; (Epub) siRNA Screening for the Small GTPase Rab Proteins Identifies that Rab5B is a Major Regulator of Hepatitis B Virus Production.

实验应用 : WB, ICC/IF

种属反应 : Human

Huang WT et al. Cancer Lett 2019; 442:287-298 Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1.

实验应用 : WB

种属反应 : Human

Kondo Y et al. JCI Insight 2018; 3 (14) Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking.

Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.

实验应用 : IP

种属反应 : Human

Fan J et al. J Cell Biol 2017; 216 (12):4153-4164 cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.

Chang HY et al. Sci Rep 2017; 7 (1):3515 Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells.

实验应用 : ICC/IF

种属反应 : Mouse

Morioka S et al. Exp Cell Res 2017; (Epub) Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.

实验应用 : WB

种属反应 : Mouse

Sugahara D et al. Glycobiology 2016; (Epub) Mouse intestinal niche cells express a distinct α1, 2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia.

Lopes da Fonseca T et al. Hum Mol Genet 2016; (Epub) A familial ATP13A2 mutation enhances alpha-synuclein aggregation and promotes cell death.

Miyauchi Y et al. Mol Pharmacol 2015; 88 (4):800-12 Suppression of Cytochrome P450 3A4 Function by UDP-Glucuronosyltransferase 2B7 through a Protein-Protein Interaction: Cooperative Roles of the Cytosolic Carboxyl-Terminal Domain and the Luminal Anchoring Region.

实验应用 : WB

种属反应 : Human

Palmer DN et al. Biochim Biophys Acta 2015; 1852 (10 Pt B):2279-86 Recent studies of ovine neuronal ceroid lipofuscinoses from BARN, the Batten Animal Research Network.

实验应用 : ICC/IF

种属反应 : Sheep

Sharma S et al. Nat Commun 2015; 6:6881 APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages.

实验应用 : WB

种属反应 : Human

De Palma C et al. Skelet Muscle 2014; 4 (1):22 Deficient nitric oxide signalling impairs skeletal muscle growth and performance: involvement of mitochondrial dysregulation.

实验应用 : WB

种属反应 : Mouse

Luo YH et al. Chem Res Toxicol 2013; 26 (5):662-73 Cadmium-based quantum dot induced autophagy formation for cell survival via oxidative stress.

实验应用 : ICC/IF

种属反应 : Mouse

Chen YT et al. PLoS One 2012; 7 (9):e46171 R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.

实验应用 : ICC/IF

种属反应 : Mouse

Holzer RG et al. Cell 2011; 147 (1):173-84 Saturated fatty acids induce c-Src clustering within membrane subdomains, leading to JNK activation.

实验应用 : WB

种属反应 : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Calnexin抗体 [C3], C-term

目录号 GTX109669

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX109669 WB Image

GTX109669 IP Image

GTX109669 WB Image

GTX109669 IHC-P Image

GTX109669 IHC-P Image

GTX109669 IHC-P Image

GTX109669 WB Image

GTX109669 WB Image

GTX109669 WB Image

GTX109669 WB Image

GTX109669 ICC/IF Image

GTX109669 IHC-P Image

GTX109669 IHC-P Image

GTX109669 IHC-P Image

GTX109669 WB Image

GTX109669 ICC/IF Image

GTX109669 IHC-P Image

GTX109669 WB Image

GTX109669 ICC/IF Image

GTX109669 WB Image

GTX109669 WB Image

文献引用

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Calnexin antibody [C3], C-term 目录号 GTX109669

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