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CD73抗体 [N1N3]

Orthogonal
Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509
Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

目录号 GTX113509

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, Neutralizing/Inhibition, PLA

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD73 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Neutralizing/Inhibition Assay dependent
PLA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

63 kDa. ( Note )

阳性对照

HepG2 , HCT116 , mouse placenta

预测种属反应

Rat, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10720009

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

细胞定位

Cell membrane , GPI-anchor

功能与背景

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

CD73 antibody [N1N3] detects CD73 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: CD73 stained by CD73 antibody [N1N3] (GTX113509) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113509

GTX113509 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
B: HCT116
12% SDS PAGE
GTX113509 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N1N3] used in Western Blot (WB). GTX113509

GTX113509 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

Nakao Y et al. Acta Biomater 2021; 122 : 306-324 Exosomes from TNF-α-treated human gingiva-derived MSCs enhance M2 macrophage polarization and inhibit periodontal bone loss.
实验应用 : Neutralizing/Inhibition
Casciaro F et al. Mech Ageing Dev 2020; 191 : 111328 Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells.
实验应用 : ICC/IF
种属反应 : Human
M?ller GA et al. Mech Ageing Dev 2020; 190 : 111307 Age-dependent membrane release and degradation of full-length glycosylphosphatidylinositol-anchored proteins in rats.
Aoki M et al. BMC Cancer 2019; 19 (1) : 912 CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.
实验应用 : PLA
种属反应 : Human
Ma SR et al. Mol Cancer 2017; 16 (1) : 99 Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma.
实验应用 : WB, IHC-P
种属反应 : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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