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CD146抗体 [C1C3]

KO/KD Orthogonal
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

目录号 GTX108777

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

72 kDa. ( Note )

阳性对照

rat liver , HUVEC , HeLa

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10729041

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

数据库

研究领域

產品图片

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

KO/KD

Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD146 stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by Western blot analysis.
A. 30 μg HUVEC whole cell lysate/extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:10000

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD146 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108777

GTX108777 ICC/IF Image

CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by western blot analysis.
A. 50 μg rat liver extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:1000

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

文献引用

Application Reference

Suardi D et al. Med Sci Monit 2023; 29 : e939125 Comparison of Extravillous Intermediate Trophoblast Invasion Depth and Distribution Pattern Between Placenta Accreta and Non-Accreta.
实验应用 : IHC-P
种属反应 : Human
Hu Y et al. J Orthop Translat 2020; 24 : 12-22 Defactinib attenuates osteoarthritis by inhibiting positive feedback loop between H-type vessels and MSCs in subchondral bone.
Pearse DD et al. Neuroscience 2020; (Epub) Neuronal and Endothelial Transglutaminase-2 Expression during Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis.
实验应用 : IHC-P
种属反应 : Rat
Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
实验应用 : WB
种属反应 : Mouse
Akiyama Y et al. Proc Natl Acad Sci U S A 2019; 116 (16) : 7738-7743 Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.
实验应用 : ICC/IF
种属反应 : Rat
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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