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CBP抗体 [C3], C-term

KO/KD Orthogonal
Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249
Anti-CBP antibody [C3], C-term used in Immunoprecipitation (IP). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249
Anti-CBP antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249
Anti-CBP antibody [C3], C-term used in ChIP assay (ChIP assay). GTX101249
Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249
Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249
Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249
Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

目录号 GTX101249

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP, ChIP assay

种属反应

 Human, Mouse, Monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CBP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

265 kDa. ( Note )

阳性对照

A549 , H1299 , HCT-116 , HeLa

预测种属反应

Rat, Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human CBP. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240560

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CREB binding protein , CBP , KAT3A , MKHK1 , RSTS , RSTS1

细胞定位

Nucleus

功能与背景

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249

GTX101249 WB Image

KO/KD

Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody [C3], C-term used in Immunoprecipitation (IP). GTX101249

GTX101249 IP Image

CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 μg of CBP antibody (GTX101249) 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody (GTX101249) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBP antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101249

GTX101249 ICC/IF Image

CBP antibody [C3], C-term detects CBP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CBP protein stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBP antibody [C3], C-term used in ChIP assay (ChIP assay). GTX101249

GTX101249 ChIP assay Image

CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of CREBBP antibody (GTX101249) The precipitated DNA was detected by PCR with primer set targeting to snail promoter.

Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249

GTX101249 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249

GTX101249 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249

GTX101249 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CBP stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6

文献引用

Application Reference

Pratiwi HM et al. Front Cell Dev Biol 2023; 11 : 1170691 Heterochronic development of pelvic fins in zebrafish: possible involvement of temporal regulation of pitx1 expression.
Chen SJ et al. Theranostics 2021; 11 (10) : 4672-4687
实验应用 : WB
种属反应 : Human
Sengupta S et al. DNA Repair (Amst) 2018; 66-67 : 1-10 Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.
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CBP antibody [C3], C-term 目录号 GTX101249

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-CBP antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101249
Date : Anonymous submitted on 07-Feb-2013
Score :
Application Tested : ICC/IF
Sample Species : Mk
Sample : Vero E6 cells
Blocking : 3% BSA, 23°C, 1Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : Acetone/DPBS
Primary Antibody : 1:200, 23°C, 2Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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