GeneTex中国官方网站
  • China

Bcl-2抗体 [N1N2], N-term

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

目录号 GTX100064

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat, Zebrafish, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
产品说明

摘要

BCL-2 antibody recognizes BCL-2 protein. BCL-2 (26 kDa) regulates mitochondrial dynamics, is involved in the regulation of mitochondrial fusion and fission, and is associated with apoptosis. Abnormal expression of BCL-2 protein is linked to various cancers, autoimmunity, and psychiatric disorders. This BCL-2 antibody is a widely published rabbit polyclonal validated for multiple applications.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

26 kDa. ( Note )

阳性对照

BCL2-transfected 293T

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036326

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

细胞定位

Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

功能与背景

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064

GTX100064 ICC/IF Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Bcl-2 antibody detects Bcl-2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
Bcl-2 stained by Bcl-2 antibody (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

PG-4 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Bcl-2 antibody detects Bcl-2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Bcl-2 stained by Bcl-2 antibody (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Biomolecules in 2020.PMID: 32635653

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal PLoS One in 2015. PMID: 25849560

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal PLoS One in 2016. PMID: 26859149

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Apoptosis in 2020. PMID: 32100210

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

The data was published in the journal Apoptosis in 2020. PMID: 32100210

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Apoptosis in 2020. PMID: 32100210

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

The data was published in the journal Apoptosis in 2020. PMID: 32100210

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31210840

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

The data was published in the journal J Cell Mol Med in 2018. PMID: 29682889

文献引用

产品评论

Bcl-2 antibody [N1N2], N-term 目录号 GTX100064

Rating ( Average 4.6 based on 5 users reviews)
实验应用
Western Blot(WB) ( Average 4.6 based on 5 users reviews)
Date : Anonymous submitted on 10-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10ug
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 16°C, 25Hr
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PA-1 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
Qi Y et al. Cancer Cell Int 2021; 21 (1):74
实验应用 : WB
种属反应 : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com