BRCA1抗体 [17F8] - ChIP grade

Name: BRCA1抗体 [17F8] - ChIP grade
Brand: GeneTex
SKU: GTX70111
Price: 4300.00 RMB

Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111

Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

Anti-BRCA1 antibody [17F8] - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX70111

目录号 GTX70111

文献引用 25

宿主	Mouse
克隆	Monoclonal
克隆编号	17F8
同种型	IgG1
实验应用	WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC
种属反应	Human, Mouse
产品包装	100 μl (¥4300)

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View product citations for antibody GTX70111 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1 μg/ml
IHC-P	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent
IHC	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

208 kDa. ( Note )

阳性对照

293T , A431 , HeLa

产品说明

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_368627

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

细胞定位

Nucleus , Cytoplasm

功能与背景

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

数据库

Gene ID: 672 BRCA1
UniProt: P38398 BRCA1

研究领域

產品图片

GTX70111 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

GTX70111 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

GTX70111 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70111 WB Image

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX70111 WB Image

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.