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Alpha fetoprotein / AFP抗体 [2A9]

Overexpression
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX84954

目录号 GTX84954

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 2A9

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, FACS

种属反应

 Human, Mouse, Dog, Monkey
产品包装
100 μl (¥7300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

69 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

纯化方式

Purified by affinity chromatography

偶联

Unconjugated

RRID

AB_10727804

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

alpha fetoprotein , AFPD , FETA , HPAFP

细胞定位

Secreted

功能与背景

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

数据库

研究领域

產品图片

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84954

GTX84954 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 5 ug per lane

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954

GTX84954 FACS Image

FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 35 ug per lane

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954

GTX84954 FACS Image

FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Red : Primary antibody
Blue : Negative control antibody

Anti-Alpha fetoprotein / AFP antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX84954

GTX84954 IHC-P Image

IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

文献引用

Application Reference

Chen YH et al. Sci Rep 2019; 9 (1) : 9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
实验应用 : ICC/IF
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 7300
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