ATG5抗体

Name: ATG5抗体
Brand: GeneTex
SKU: GTX113309
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309

目录号 GTX113309

文献引用 15

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, PLA
种属反应	Human, Mouse
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX113309 on CiteAb

产品说明

摘要

ATG5 antibody detects autophagy related 5 (ATG5), a ~32kDa key protein that contributes to the formation of autophagic vesicles when conjugated to ATG12 through the actions of ATG7 and ATG10. It plays key roles in mitochondrial function, lymphocyte biology, and axonal maintenance, among others. Dysregulation of ATG5 is associated with several diseases, including sporadic Parkinson's disease, childhood asthma, and cancers.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
PLA	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

32 kDa. ( Note )

阳性对照

NT2D1 , PC-3 , HeLa (chloroquine treatment for 24hr)

预测种属反应

Bovine, Chicken, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10633458

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

细胞定位

Cytoplasm

数据库

Gene ID: 9474 ATG5
UniProt: Q9H1Y0 ATG5

研究领域

產品图片

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

GTX113309 WB Image

ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113309 IHC-P Image

ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ATG5 antibody (GTX113309) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX113309 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: PC-3
12% SDS PAGE
GTX113309 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

文献引用

Application Reference

Li J et al. iScience 2023; 26 (1) : 105858 Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury.

Tiezhi Li et al. Cancer Cell Int 2022; 22 (1) : 120

实验应用 : WB

种属反应 : Human

Tianjiao Zeng et al. Oncol Rep 2021; 46 (1)

实验应用 : WB

种属反应 : Human

Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.

实验应用 : WB

种属反应 : Human

Yang CC et al. Antioxidants (Basel) 2021; 10 (6) Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice.

实验应用 : WB

种属反应 : Mouse

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产品评论

ATG5 antibody 目录号 GTX113309

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 18-Dec-2023

Score :

Application Tested : WB

Sample Species : Hu

Sample : HT29 and HCT116 cells

Amount used : 10μg

Blocking : 5% non-fat milk in TBST, 4°C, 1Hr

Primary Antibody : 1:1000, 4°C, 15Hr

Date : Anonymous submitted on 06-May-2021

Score :

Application Tested : WB

Sample Species : Ms

Sample : MEF cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST, 27°C, 1Hr

Primary Antibody : 1:5000, 4°C, 12Hr

Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr

条带说明 : Two groups (DMEM and CM) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

我要评论产品

Application Reference

Li J et al. iScience 2023; 26 (1):105858 Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury.

Tiezhi Li et al. Cancer Cell Int 2022; 22 (1):120

实验应用 : WB

种属反应 : Human

Tianjiao Zeng et al. Oncol Rep 2021; 46 (1)

实验应用 : WB

种属反应 : Human

Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.

实验应用 : WB

种属反应 : Human

实验应用 : WB

种属反应 : Mouse

Ha? A et al. Cells 2021; 10 (4) S6K1 Is Indispensible for Stress-Induced Microtubule Acetylation and Autophagic Flux.

实验应用 : WB

种属反应 : Mouse

Nowosad A et al. Cell Death Dis 2021; 12 (5):481 p27 controls autophagic vesicle trafficking in glucose-deprived cells via the regulation of ATAT1-mediated microtubule acetylation.

Lin TY et al. Autophagy 2019;:1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

实验应用 : PLA

种属反应 : Human

Wong CW et al. J Med Chem 2019; 62 (15):7111-7125 Toward Understanding the Antitumor Effects of Water-Soluble Fullerene Derivatives on Lung Cancer Cells: Apoptosis or Autophagy Pathways?

实验应用 : WB

种属反应 : Human

Li FY et al. Glycobiology 2018; (Epub) Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner.

实验应用 : WB

种属反应 : Human

Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.

实验应用 : WB

种属反应 : Human

Koh EH et al. J Biol Chem 2016; 291 (47):24747-24755 Mitochondrial Activity in Human White Adipocytes Is Regulated by the Ubiquitin Carrier Protein 9/microRNA-30a Axis.

实验应用 : WB

种属反应 : Human

Hsieh YY et al. Am J Cancer Res 2016; 6 (8):1661-80 EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.

实验应用 : WB

种属反应 : Human

Lo MC et al. Am J Physiol Endocrinol Metab 2015; 309 (10):E829-39 nε-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased * secretion from β-cells.

实验应用 : WB

种属反应 : Mouse

Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

实验应用 : WB

种属反应 : Human

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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ATG5抗体

目录号 GTX113309

宿主

克隆

同种型

实验应用

种属反应

摘要

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

数据库

研究领域

產品图片

GTX113309 ICC/IF Image

GTX113309 WB Image

GTX113309 IHC-P Image

GTX113309 ICC/IF Image

GTX113309 WB Image

GTX113309 WB Image

GTX113309 WB Image

GTX113309 WB Image

GTX113309 WB Image

文献引用

Application Reference

产品评论

ATG5 antibody 目录号 GTX113309

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