AChE抗体

Name: AChE抗体
Brand: GeneTex
SKU: GTX101648
Price: 4000.00 RMB

Anti-AChE antibody used in IHC (Frozen sections) (IHC-Fr). GTX101648

Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648

Anti-AChE antibody used in Western Blot (WB). GTX101648

Anti-AChE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101648

目录号 GTX101648

文献引用 1

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX101648 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

Raji , mouse brain , rat brain

预测种属反应

Bovine, Cat, Guinea pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.81 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949564

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT

细胞定位

Cell junction , synapse , Secreted , Cell membrane; Peripheral membrane protein , Isoform T: Nucleus , Isoform H: Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Cell junction , synapse , Secreted , Cell membrane , Isoform T: Nucleus , Isof

功能与背景

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]

数据库

Gene ID: 43 ACHE
UniProt: P22303 ACHE

研究领域

產品图片

GTX101648 IHC-Fr Image

AChE antibody detects AChE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: AChE protein stained by AChE antibody (GTX101648) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AChE stained by AChE antibody (GTX101648) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101648 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500.

GTX101648 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101648 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101648 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101648 ICC/IF Image

AChE antibody detects AChE protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody (GTX101648) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101648 IHC-P Image

AChE antibody detects AChE protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
AChE antibody (GTX101648) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101648 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE(GTX101648) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX101648 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101648 WB Image

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
AChE stained by AChE antibody (GTX101648) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101648 IHC-P Image

AChE antibody detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AChE stained by AChE antibody (GTX101648) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

El-Mehi AE et al. Ann Anat 2019; : 151428 Controlled ozone therapy modulates the neurodegenerative changes in the frontal cortex of the aged albino rat.

实验应用 : IHC-P

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Application Reference

El-Mehi AE et al. Ann Anat 2019;:151428 Controlled ozone therapy modulates the neurodegenerative changes in the frontal cortex of the aged albino rat.

实验应用 : IHC-P

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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AChE抗体

目录号 GTX101648

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX101648 IHC-Fr Image

GTX101648 IHC-P Image

GTX101648 WB Image

GTX101648 WB Image

GTX101648 IHC-P Image

GTX101648 WB Image

GTX101648 ICC/IF Image

GTX101648 IHC-P Image

GTX101648 IHC-P Image

GTX101648 ICC/IF Image

GTX101648 WB Image

GTX101648 WB Image

GTX101648 IHC-P Image

GTX101648 IHC-P Image

文献引用

Application Reference

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